Table 2

Prevalence of Herpes B Virus in Wild Long-Tailed Macaques, Thailand, 2018–2024

Krittiga Sapkanarak, Jiro Yasuda, Murasaki Amano, Suthirote Meesawat, Taratorn Kemthong, Titiporn Kaikaew, and Suchinda Malaivijitnond

Author affiliation: Chulalongkorn University Faculty of Science, Bangkok, Thailand (K. Sapkanarak, T. Kaikaew, S. Malaivijitnond); Nagasaki University, Nagasaki, Japan (J. Yasuda, M. Amano); National Primate Research Center of Thailand, Saraburi, Thailand (S. Meesawat, T. Kemthong, S. Malaivijitnond)

Main Article

PCR primers specific for B virus sequences in study of prevalence of herpes B virus in wild long-tailed macaques, Thailand, 2018–2024*

Primer set	Primer names	Primer sequences, 5′ → 3′
UL27, first PCR	UL27_F1	ACGTCATCATGCAGAACTCG
UL27, first PCR	UL27_R1	GGTTGGAGAGGAAGGAGGAC
UL27, second PCR	UL27_F2	ACGTCATCATGCAGAACTCG
UL27, second PCR	UL27_R2	GGCCCTCGAAGAAGGAGTAG
UL29, first PCR	UL29_F1	CAGTAGCGCAGGATCTGGTTG
UL29, first PCR	UL29_R1	GCGTGATGGACCTCTTYAACA
UL29, second PCR	UL29_F2	CAGGATCTGGTTGGCCATGTAG
UL29, second PCR	UL29_R2	GCGTGATGGACCTCTTYAACA
US5-US6, first PCR	US5-US6_F1	CGTTTCCTCCCGTGGACTTC
US5-US6, first PCR	US5-US6_R1	GTCTGGAACGGGTTCTCCAC
US5-US6, second PCR	US5-US6_F2	CTGACCCTGGCCGCCATG
US5-US6, second PCR	US5-US6_R2	GGTCCGTCTTCTGCTCCAGCG

*Primers were designed to detect conserved regions within the UL27 and UL29 genes and confirm the positive cases identified by using quantitative PCR and loop-mediated isothermal amplification and to detect hypervariable regions (US5-US6 genes) for phylogenetic analysis.

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Page created: February 08, 2025

Page updated: March 24, 2025

Page reviewed: March 24, 2025

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