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Volume 31, Number 4—April 2025
Research

Prevalence of Herpes B Virus in Wild Long-Tailed Macaques, Thailand, 2018–2024

Krittiga Sapkanarak, Jiro Yasuda, Murasaki Amano, Suthirote Meesawat, Taratorn Kemthong, Titiporn Kaikaew, and Suchinda Malaivijitnond
Author affiliation: Chulalongkorn University, Bangkok, Thailand (K. Sapkanarak, T. Kaikaew, S. Malaivijitnond); Nagasaki University, Nagasaki, Japan (J. Yasuda, M. Amano); National Primate Research Center of Thailand, Saraburi, Thailand (S. Meesawat, T. Kemthong, S. Malaivijitnond)

Main Article

Table 5

Detection of B virus DNA by using qPCR and LAMP in wild long-tailed macaques in study of prevalence of herpes B virus in Thailand, 2018–2024*

Sample no. Age class Sex qPCR of UL29 gene
LAMP of UL27 gene
Average Ct Estimated DNA copy no. Amplification time, h:m:s Annealing temperature, °C
WTT47 Juvenile M 37.5 9 0 87.16
WKN63 Subadult M 36.3 20 0:24:31 89.97
WKN64 Juvenile F 27.1 5,565 0:14:16 90.21
WKN73 Subadult F 27.1 5,672 0:14:31 90.35
BTB14 Subadult M 29.6 1,199 0:15:46 90.36
CAO38 Subadult F 38.0 7 0:24:46 90.21
CAO41 Subadult M 26.7 7,074 0:15:01 90.36
TPK5 Juvenile M 32.1 1,607 0:15:31 90.36
MFRC17 Adult M 25.7 12,579 0:13:16 90.01

*Ct, cycle threshold; h:m:s, hours:minutes:seconds; LAMP, loop-mediated isothermal amplification; qPCR, quantitative PCR.

Main Article

Page created: February 08, 2025
Page updated: March 07, 2025
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