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Volume 31, Number 4—April 2025
Research

Neutralizing Antibodies against California Serogroup Orthobunyaviruses in Human Serum Samples, Montana, USA

Tyson A. Woods1, Stephen F. Johnson1, Alyssa B. Evans2, and Karin E. PetersonComments to Author 
Author affiliation: Laboratory of Neurological Infections and Immunity, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, Montana, USA

Main Article

Figure 5

Cross-reactivity between JCV and CEV NAb responses in mice and humans in a study of neutralizing antibodies against California serogroup orthobunyaviruses in human serum samples, Montana, USA. A) Mouse inoculation at 5-fold dilution. Plasma from mice infected with JCV or CEV at 3 weeks postinfection were analyzed for the ability to neutralize JCV or CEV by using 5-fold dilutions of plasma. Each symbol represents an individual mouse with the highest dilution that neutralized 50% of virus. B) Comparison of mouse plasma and human serum samples analyzed at 2-fold dilutions for JCV and CEV responses. Plasma samples were from the same set of mice; sample symbols and dotted lines connect for each virus. Four human serum samples that were dual positive for high titers of JCV and CEV at 1:1,250 (Appendix Figure 1) were analyzed at 2-fold dilution. Samples with p values were at the borderline of 50% inhibition between 1:640 and 1:320. For each set, data were analyzed by a paired t-test for differences in the NAb response between JCV NAb and CEV NAb. CEV, California encephalitis virus; IP, intraperitoneal; JCV, Jamestown Canyon virus; NAb, neutralizing antibodies.

Figure 5. Cross-reactivity between JCV and CEV NAb responses in mice and humans in a study of neutralizing antibodies against California serogroup orthobunyaviruses in human serum samples, Montana, USA. A) Mouse inoculation at 5-fold dilution. Plasma from mice infected with JCV or CEV at 3 weeks postinfection were analyzed for the ability to neutralize JCV or CEV by using 5-fold dilutions of plasma. Each symbol represents an individual mouse with the highest dilution that neutralized 50% of virus. B) Comparison of mouse plasma and human serum samples analyzed at 2-fold dilutions for JCV and CEV responses. Plasma samples were from the same set of mice; sample symbols and dotted lines connect for each virus. Four human serum samples that were dual positive for high titers of JCV and CEV at 1:1,250 (Appendix Figure 1) were analyzed at 2-fold dilution. Samples with p values were at the borderline of 50% inhibition between 1:640 and 1:320. For each set, data were analyzed by a paired t-test for differences in the NAb response between JCV NAb and CEV NAb. CEV, California encephalitis virus; IP, intraperitoneal; JCV, Jamestown Canyon virus; NAb, neutralizing antibodies.

Main Article

1These first authors contributed equally to this article.

2Current affiliation: Department of Microbiology and Cell Biology, Montana State University, Bozeman, Montana, USA

Page created: February 08, 2025
Page updated: March 24, 2025
Page reviewed: March 24, 2025
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