Skip directly to site content Skip directly to page options Skip directly to A-Z link Skip directly to A-Z link Skip directly to A-Z link

Disclaimer: Early release articles are not considered as final versions. Any changes will be reflected in the online version in the month the article is officially released.

Volume 31, Number 5—May 2025
Research

Metagenomic Identification of Fusarium solani Strain as Cause of US Fungal Meningitis Outbreak Associated with Surgical Procedures in Mexico, 2023

Charles Y. ChiuComments to Author , Venice Servellita, Mikael de Lorenzi-Tognon, Patrick Benoit, Nanami Sumimoto, Abiodun Foresythe, Filipe M. Cerqueira, Natalie Williams-Bouyer, Ping Ren, Lauren Nicholas S. Herrera, David C. Gaston, Leanna Sayyad, Shannon L. Whitmer, John Klena, Holenarasipur R. Vikram, Jeremy A.W. Gold, Lalitha Gade, Lindsay Parnell, Elizabeth Misas, Tom M. Chiller, Isabel S. Griffin, Sridhar V. Basavaraju, Dallas J. Smith, Anastasia P. Litvintseva, and Nancy A. Chow
Author affiliation: Chan-Zuckerberg Biohub, San Francisco, California, USA (C.Y. Chiu); University of California San Francisco, San Francisco, California, USA (C.Y. Chiu, V. Servellita, M. de Lorenzi-Tognon, P. Benoit, N. Sumimoto, A. Foresythe); Abbott Pandemic Defense Coalition, Abbott Park, Illinois, USA (C.Y. Chiu, V. Servellita, M. de Lorenzi-Tognon, P. Benoit, N. Sumimoto, A. Foresythe); The University of Texas Medical Branch at Galveston, Galveston, Texas, USA (F.M. Cerqueira, N. Williams-Bouyer, P. Ren); Vanderbilt University Medical Center, Nashville, Tennessee, USA (L.N.S. Herrera, D.C. Gaston); Centers for Disease Control and Prevention, Atlanta, Georgia, USA (L. Sayyad, S.L. Whitmer, J. Klena, J.A.W. Gold, L. Gade, L. Parnell, E. Misas, T.M. Chiller, I.S. Griffin, S.V. Basavaraju, D.J. Smith, A.P. Litvintseva, N.A. Chow); Mayo Clinic, Phoenix, Arizona, USA (H.R. Vikram)

Main Article

Figure 2

Flowchart showing the metaMELT analysis workflow used for identification of Fusarium solani strain as cause of fungal meningitis US outbreak associated with surgical procedures in Mexico, 2023. metaMELT (metagenomic multiple extended locus typing), is a novel analytic technique for simultaneously diagnosing the infection and characterizing the interrelatedness of F. solani strains. metaMELT uses the following steps: A) perform mNGS analysis of patient samples (i.e., cerebrospinal fluid, plasma, or brain tissue), using the SURPI+ computational pipeline (https://github.com/chiulab/SURPI-plus-dist) to identify pathogens; B) identify F. solani reads; C) map reads to the F. solani reference genome and then extract and concatenate; D) perform phylogenetic analysis on concatenated sequences. SURPI, sequence-based ultra-rapid pathogen identification.

Figure 2. Flowchart showing the metaMELT analysis workflow used for identification of Fusarium solani strain as cause of fungal meningitis US outbreak associated with surgical procedures in Mexico, 2023. metaMELT (metagenomic multiple extended locus typing), is a novel analytic technique for simultaneously diagnosing the infection and characterizing the interrelatedness of F. solani strains. metaMELT uses the following steps: A) perform mNGS analysis of patient samples (i.e., cerebrospinal fluid, plasma, or brain tissue), using the SURPI+ computational pipeline (https://github.com/chiulab/SURPI-plus-dist) to identify pathogens; B) identify F. solani reads; C) map reads to the F. solani reference genome and then extract and concatenate; D) perform phylogenetic analysis on concatenated sequences. SURPI, sequence-based ultra-rapid pathogen identification.

Main Article

Page created: March 21, 2025
Page updated: April 03, 2025
Page reviewed: April 03, 2025
The conclusions, findings, and opinions expressed by authors contributing to this journal do not necessarily reflect the official position of the U.S. Department of Health and Human Services, the Public Health Service, the Centers for Disease Control and Prevention, or the authors' affiliated institutions. Use of trade names is for identification only and does not imply endorsement by any of the groups named above.
file_external