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Volume 31, Number 5—May 2025
Research

Metagenomic Identification of Fusarium solani Strain as Cause of US Fungal Meningitis Outbreak Associated with Surgical Procedures in Mexico, 2023

Charles Y. ChiuComments to Author , Venice Servellita, Mikael de Lorenzi-Tognon, Patrick Benoit, Nanami Sumimoto, Abiodun Foresythe, Filipe M. Cerqueira, Natalie Williams-Bouyer, Ping Ren, Lauren Nicholas S. Herrera, David C. Gaston, Leanna Sayyad, Shannon L. Whitmer, John Klena, Holenarasipur R. Vikram, Jeremy A.W. Gold, Lalitha Gade, Lindsay Parnell, Elizabeth Misas, Tom M. Chiller, Isabel S. Griffin, Sridhar V. Basavaraju, Dallas J. Smith, Anastasia P. Litvintseva, and Nancy A. Chow
Author affiliation: Chan-Zuckerberg Biohub, San Francisco, California, USA (C.Y. Chiu); University of California San Francisco, San Francisco (C.Y. Chiu, V. Servellita, M. de Lorenzi-Tognon, P. Benoit, N. Sumimoto, A. Foresythe); Abbott Pandemic Defense Coalition, Abbott Park, Illinois, USA (C.Y. Chiu, V. Servellita, M. de Lorenzi-Tognon, P. Benoit, N. Sumimoto, A. Foresythe); The University of Texas Medical Branch at Galveston, Galveston, Texas, USA (F.M. Cerqueira, N. Williams-Bouyer, P. Ren); Vanderbilt University Medical Center, Nashville, Tennessee, USA (L.N.S. Herrera, D.C. Gaston); Centers for Disease Control and Prevention, Atlanta, Georgia, USA (L. Sayyad, S.L. Whitmer, J. Klena, J.A.W. Gold, L. Gade, L. Parnell, E. Misas, T.M. Chiller, I.S. Griffin, S.V. Basavaraju, D.J. Smith, A.P. Litvintseva, N.A. Chow); Mayo Clinic, Phoenix, Arizona, USA (H.R. Vikram)

Main Article

Figure 5

Effect of number of reference genomes on performance of metaMELT (metagenomic multiple extended locus typing, a novel analytic technique for simultaneously diagnosing the infection and characterizing the interrelatedness of Fusarium solani strains) for identification of F. solani strain as cause of fungal meningitis US outbreak associated with surgical procedures in Mexico, 2023. A, B) metaMELT phylogenetic trees that include mNGS reads from patients P1–P4 are shown with and without outbreak-related genomes: A) only 1 outbreak-related reference genome from P5; B) tree without any outbreak-related reference genomes. Note the clustering of patients P1–P4 (pink shaded regions) even in the absence of an outbreak-related reference genome. C, D) metaMELT phylogenetic trees that include mNGS reads from patients P1–P4 are shown with only 10 reference genomes, including patient P5 (C); and only 5 reference genomes, including patient P5 (D). Scale bars indicate nucleotide substitutions per site. P1–P5, patients 1­–5.

Figure 5. Effect of number of reference genomes on performance of metaMELT (metagenomic multiple extended locus typing, a novel analytic technique for simultaneously diagnosing the infection and characterizing the interrelatedness of Fusarium solani strains) for identification of F. solani strain as cause of fungal meningitis US outbreak associated with surgical procedures in Mexico, 2023. A, B) metaMELT phylogenetic trees that include mNGS reads from patients P1–P4 are shown with and without outbreak-related genomes: A) only 1 outbreak-related reference genome from P5; B) tree without any outbreak-related reference genomes. Note the clustering of patients P1–P4 (pink shaded regions) even in the absence of an outbreak-related reference genome. C, D) metaMELT phylogenetic trees that include mNGS reads from patients P1–P4 are shown with only 10 reference genomes, including patient P5 (C); and only 5 reference genomes, including patient P5 (D). Scale bars indicate nucleotide substitutions per site. P1–P5, patients 1­–5.

Main Article

Page created: March 21, 2025
Page updated: April 25, 2025
Page reviewed: April 25, 2025
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