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Volume 31, Number 8—August 2025

Dispatch

Identification of Co-Circulating Dengue and South America–Origin Zika Viruses, Pakistan, 2021–2022

Najeeha T. Iqbal1, Kaitlin Sawatzki1, Kumail Ahmed, Jennifer Tisoncik-Go, Elise Smith, Kathleen Voss, John Cornelius, Lu Wang, Alicen B. Spaulding, Leonid Serebryannyy, Daniel C. Douek, Muhammad Asif Syed, Syed Faisal Mahmood, Erum Khan, Wesley C. Van Voorhis, and Michael GaleComments to Author 
Author affiliation: The Aga Khan University, Karachi, Pakistan (N.T. Iqbal, K. Ahmed, S.F. Mahmood, E. Khan); University of Washington, Seattle, Washington, USA (K. Sawatzki, J. Tisoncik-Go, E. Smith, K. Voss, J. Cornelius, W.C. Van Voorhis); National Institutes of Health, National Institute of Allergy and Infectious Diseases, Bethesda, Maryland, USA (L. Wang, A.B. Spaulding, L. Serebryannyy, D.C. Douek); Pakistan Field Epidemiology Laboratory Training Program, Karachi (M.A. Syed); University of Minnesota, Minneapolis, Minnesota, USA (M. Gale, Jr.)

Main Article

Table 1

Real time qRT-PCR results for DENV and ZIKV in metagenomics analysis groups for co-circulation of dengue and South America–origin Zika viruses, Pakistan, 2021–2022

Patient DENV (Ct<40) ZIKV (Ct<40) ZIKV (Ct<38.5) ZIKV (Ct<38.5)
A 20.3 Negative Not done Not done
B 39.74 Negative Not done Not done
C 26.91 Negative Not done Not done
D 31.74 Negative Not done Not done
E 37.77 38.73 Not done Not done
F 39.35 Negative 37.33 39.05
G Not done Not done Not done Not done

*Bold text represents positive assay values. Ct, cycle threshold; DENV, dengue virus; qRT-PCR, quantitative reverse transcription PCR; ZIKV, Zika virus.

Main Article

1These first authors contributed equally to this article.

Page created: July 02, 2025
Page updated: July 22, 2025
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