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Volume 31, Number 9—September 2025

Research Letter

Monkeypox Virus Clade IIa Infections, Liberia, 2023–2024

Dougbeh Chris Nyan1, Irina Maljkovic Berry1, Bode I. Shobayo, Monika Mehta, Gabriella Worwa, Shawn Hirsch, Sarah E. Klim, Fahn Taweh, Kalilu S. Donzo, B.M. Freeman, Alberta B. Corvah, Francis Jaryan, Julius S.M. Gilayeneh, Ian Crozier, Courtney Renken, Laura McNay, J. Soka Moses, Jens H. Kuhn, Lisa E. Hensley, and Sara E. ZufanComments to Author 
Author affiliation: National Public Health Institute of Liberia, Monrovia, Liberia (D.C. Nyan, B.I. Shobayo, F. Taweh, K.S. Donzo, B.M. Freeman, A.B. Corvah, F. Jaryan, J.S.M. Gilayeneh, J.S. Moses); National Institutes of Health, Frederick, Maryland, USA (I. Maljkovic Berry, M. Mehta, G. Worwa, S. Hirsch, S.E. Klim, J.H. Kuhn, S.E. Zufan); Clinical Monitoring Research Program Directorate, Frederick National Laboratory for Cancer Research, Frederick (I. Crozier); National Institutes of Health, Bethesda, Maryland, USA (C. Renken, L. McNay); US Department of Agriculture, Manhattan, Kansas, USA (L.E. Hensley)

Main Article

Table

Patient and sample characteristics from suspected mpox cases in monkeypox virus clade IIa infections, Liberia, 2023–2024*

Sample ID Age, y/sex Location Symptom onset date Sample collection date Liferiver MPXV result Liferiver MPXV (FAM) Ct
LIB-MPV24-001 22/F Nimba 2023 Dec 28 2024 Jan 9 + 36.74
LIB-MPV24-007 5/M Sinoe 2024 Feb 8 2024 Feb 12 + 27.92
LIB-MPV24-014 30/M Nimba 2024 Mar 8 2024 Mar 12 + 37.06
LIB-MPV24-015 39/F Nimba 2024 Mar 10 2024 Mar 11 + 37.56
LIB-MPV24-027 24/F Grand Kru 2024 May 2 2024 May 6 + 37.63
LIB-MPV24-037 7/M Nimba 2024 Jun 26 2024 Jun 4 + 34.27
LIB-MPV24-054 7/F Sinoe 2024 Aug 20 2024 Aug 24 + 29.98
LIB-MPV24-069 2/M Lofa 2024 Aug 27 2024 Aug 29 + 30.33
LIB-MPV24-087 27/F Sinoe 2024 Sep 4 2024 Sep 5 + 26.44
LIB-MPV24-107 6/M River Gee 2024 Aug 17 2024 Sep 7 + 36.8
LIB-MPV24-114 20/M Bong 2024 Sep 3 2024 Sep 11 38.5
LIB-MPV24-116 16/M Bong 2024 Sep 4 2024 Sep 11 Undetermined
LIB-MPV24-117 12/M Lofa 2024 Sep 3 2024 Sep 9 Undetermined
LIB-MPV24-118 16/F Grand Gedeh 2024 Aug 25 2024 Sep 10 Undetermined
LIB-MPV24-119 56/M Montserrado 2024 Sep 10 2024 Sep 13 Undetermined
LIB-MPV24-123 29/M Bong 2024 Sep 3 2024 Sep 14 Undetermined
LIB-MPV24-136 28/M Montserrado 2024 Aug 31 2024 Sep 16 Undetermined
LIB-MPV24-141 23/M Sinoe 2024 Sep 10 2024 Sep 13 Undetermined
LIB-MPV24-142 17/F Grand Gedeh 2024 Aug 24 2024 Sep 14 Undetermined
LIB-MPV24-143 58/F Grand Kru 2024 Aug 30 2024 Sep 13 Undetermined
LIB-MPV24-144 1/M Sinoe 2024 Sep 10 2024 Sep 13 Undetermined

*Sample IDs in italics yielded near complete genomes (>98%), and sample IDs in bold met the threshold for detectable MPXV reads, defined as >1 read per kilobase of the MPXV reference genome per 1 million filtered reads. Of the 5 PCR-positive samples reported by the National Public Health Institute of Liberia that did not yield sequences, 3 had undetectable MPXV reads and insufficient DNA input (<10  ng), and 2 had detectable reads but lacked sufficient coverage for consensus genome generation. +, positive; −, negative; Ct, cycle threshold; FAM, fluorescein; MPXV, monkeypox virus.

Main Article

1These first authors contributed equally to this article.

Page created: July 10, 2025
Page updated: August 04, 2025
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