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Volume 32, Number 10—October 2026

Research

Detection of Influenza D Virus using Reverse Transcription Loop-Mediated Isothermal Amplification

Carlos Abelardo dos Santos, Jialu Li, Pauline M. van Diemen, Andrew McMahon, Benjamin C. Mollett, Andrew M. Ramsay, Meshach M. Maina, Joe James, Helen E. Everett, Janet M. Daly, and Nicole C. RobbComments to Author 
Author affiliation: Warwick Medical School, University of Warwick, Coventry, UK (C.A. dos Santos, J. Li, N.C. Robb); Animal and Plant Health Agency Weybridge, Addlestone, UK (P.M. van Diemen, B.C. Mollett, A.M. Ramsay, J. James, H.E. Everett); School of Life Sciences, University of Warwick, Coventry (A. McMahon); One Virology, Wolfson Centre for Global Virus Research, University of Nottingham, Nottingham, UK (M.M. Maina, J.M. Daly)

Main Article

Figure 2

Reverse transcription loop-mediated isothermal amplification (RT-LAMP) primers targeting the influenza D virus polymer basic protein 2 gene in study of isothermal detection of influenza D virus. All available influenza D virus polymer basic protein 2 gene sequences in the National Center for Biotechnology Information Nucleotide database (https://www.ncbi.nlm.nih.gov/nucleotide) were aligned and mutations were annotated. To improve coverage, primers were synthesized with degenerate nucleotides in positions with heterogeneity in the sequences. The consensus sequence and sequences of the 3 isolates (D/bovine/France/5920/2014, D/swine/Oklahoma/1334/2011, and D/swine/England/126471/2023) are shown for comparison. Primer target sequences are presented within the boxes, and the direction of binding is represented by the arrows.

Figure 2. Reverse transcription loop-mediated isothermal amplification (RT-LAMP) primers targeting the influenza D virus polymer basic protein 2 gene in study of isothermal detection of influenza D virus. All available influenza D virus polymer basic protein 2 gene sequences in the National Center for Biotechnology Information Nucleotide database (https://www.ncbi.nlm.nih.gov/nucleotide) were aligned and mutations were annotated. To improve coverage, primers were synthesized with degenerate nucleotides in positions with heterogeneity in the sequences. The consensus sequence and sequences of the 3 isolates (D/bovine/France/5920/2014, D/swine/Oklahoma/1334/2011, and D/swine/England/126471/2023) are shown for comparison. Primer target sequences are presented within the boxes, and the direction of binding is represented by the arrows.

Main Article

Page created: August 13, 2026
Page updated: September 28, 2026
Page reviewed: September 28, 2026
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