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Volume 32, Number 10—October 2026

Research

Isothermal Detection of Influenza D Virus using Reverse Transcription Loop-Mediated Isothermal Amplification

Carlos Abelardo dos Santos, Jialu Li, Pauline M. van Diemen, Andrew McMahon, Benjamin C. Mollett, Andrew M. Ramsay, Meshach M. Maina, Joe James, Helen E. Everett, Janet M. Daly, and Nicole C. RobbComments to Author 
Author affiliation: Warwick Medical School, University of Warwick, Coventry, UK (C.A. dos Santos, J. Li, N.C. Robb); Animal and Plant Health Agency Weybridge, Addlestone, UK (P.M. van Diemen, B.C. Mollett, A.M. Ramsay, J. James, H.E. Everett); School of Life Sciences, University of Warwick, Coventry (A. McMahon); One Virology, Wolfson Centre for Global Virus Research, University of Nottingham, Nottingham, UK (M.M. Maina, J.M. Daly)

Main Article

Figure 5

Sensitivity of RT-LAMP assay in relation to qRT-PCR Cq in study of isothermal detection of influenza D virus using RT-LAMP. A) Detection of spiked samples detected by RT-LAMP in relation to the Cq value. B) Each true positive sample was sorted according to its Cq value and RT-LAMP result. Yellow lines along the top line were positive by both RT-LAMP and qRT-PCR, pink lines on the lower line were positive by qRT-PCR but negative by RT-LAMP. The sensitivity of each range is presented as percentages at the top of the graph. Cq, cycle quantification; RT-LAMP, reverse transcription loop-mediated isothermal amplification; qRT-PCR, quantitative reverse transcription PCR.

Figure 5. Sensitivity of RT-LAMP assay in relation to qRT-PCR Cq in study of isothermal detection of influenza D virus using RT-LAMP. A) Detection of spiked samples detected by RT-LAMP in relation to the Cq value. B) Each true positive sample was sorted according to its Cq value and RT-LAMP result. Yellow lines along the top line were positive by both RT-LAMP and qRT-PCR, pink lines on the lower line were positive by qRT-PCR but negative by RT-LAMP. The sensitivity of each range is presented as percentages at the top of the graph. Cq, cycle quantification; RT-LAMP, reverse transcription loop-mediated isothermal amplification; qRT-PCR, quantitative reverse transcription PCR.

Main Article

Page created: August 13, 2026
Page updated: September 21, 2026
Page reviewed: September 21, 2026
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