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Volume 32, Number 5—May 2026

Dispatch

Tropism and Replication Competence of Cattle Influenza A(H5N1) Genotype B3.13 Virus in Human Bronchus and Lung Tissue

Kenrie P.Y. Hui, John C.W. Ho, Ka-Chun Ng, Richard J. Webby, Malik Peiris, John M. Nicholls, and Michael C.W. ChanComments to Author 
Author affiliation: Centre for Immunology & Infection, Hong Kong, China (K.P.Y. Hui, J.C.W. Ho, M. Peiris, M.C.W. Chan); School of Public Health, Li Ka Shing Faculty of Medicine, University of Hong Kong, Hong Kong (K.P.Y. Hui, K.-C. Ng, M. Peiris, M.C.W. Chan); St. Jude Children’s Research Hospital, Memphis, Tennessee, USA (R.J. Webby); School of Clinical Medicine, Li Ka Shing Faculty of Medicine, University of Hong Kong, Hong Kong (J.M. Nicholls)

Main Article

Figure 1

Viral replication kinetics in a study of ex vivo tropism and replication competence of cattle influenza A(H5N1) genotype B3.13 virus in human bronchus and lung tissue. A, B) Mean virus titers for bronchus tissues; C, D) mean virus titers for lung tissues. A, C) Culture supernatants of the infected human respiratory tissue explants were harvested at 1, 24, and 48 hours postinfection and viral titers determined by TCID50 assays; dashed line indicates assay detection limit. Statistical significance was calculated by using 2-way analysis of variance with Tukey post-hoc test. Error bars indicate SD. B, D) AUC values calculated from influenza virus titers 24–48 hours postinfection are shown. Statistical significance was calculated by using 1-way analysis of variance with Tukey post-hoc test. Error bars indicate SD. AUC, area under the curve; TCID50, 50% tissue culture infectious dose.

Figure 1. Viral replication kinetics in a study of ex vivo tropism and replication competence of cattle influenza A(H5N1) genotype B3.13 virus in human bronchus and lung tissue. A, B) Mean virus titers for bronchus tissues; C, D) mean virus titers for lung tissues. A, C) Culture supernatants of the infected human respiratory tissue explants were harvested at 1, 24, and 48 hours postinfection and viral titers determined by TCID50 assays; dashed line indicates assay detection limit. Statistical significance was calculated by using 2-way analysis of variance with Tukey post-hoc test. Error bars indicate SD. B, D) AUC values calculated from influenza virus titers 24–48 hours postinfection are shown. Statistical significance was calculated by using 1-way analysis of variance with Tukey post-hoc test. Error bars indicate SD. AUC, area under the curve; TCID50, 50% tissue culture infectious dose.

Main Article

Page created: March 24, 2026
Page updated: April 01, 2026
Page reviewed: April 01, 2026
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