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Volume 32, Number 5—May 2026
Dispatch
Tropism and Replication Competence of Cattle Influenza A(H5N1) Genotype B3.13 Virus in Human Bronchus and Lung Tissue
Table 1
Effects of desialylation on virus hemagglutination in a study of ex vivo tropism and replication competence of cattle influenza A(H5N1) genotype B3.13 virus in human bronchus and lung tissue*
| Influenza virus strain | Desialylation treatments |
|||
|---|---|---|---|---|
| Untreated | Sialidase S | Sialidase C | Sialidase A | |
| A(H1N1)pdm09 | ||||
| A/Hong Kong/415742/2009 |
16 |
16 |
0 |
0 |
| Human A(H5N1) | ||||
| A/Hong Kong/483/1997 | 8 | 0 | 0 | 0 |
| Cattle A(H5N1) | ||||
| A/dairy_cow/Ohio/B24OSU-439/2024 | 8 | 8 | 0 | 0 |
| A/dairy_cow/Texas/98638/2024 | 8 | 8 | 0 | 0 |
*Studies performed in 0.5% turkey red blood cells. The reciprocal of hemagglutination titers is denoted. Experiments were performed in triplicate and led to identical results. Substrate specificity of sialidases (all Agilent, https://agilent.com) are as follows: α(2,3)-linked sialic acid for Sialidase S; α(2,3)- and α(2–6)-linked sialic acids for Sialidase C; and α(2,3)-linked, α(2–6)-linked, α(2–8)-linked, and α(2–9)-linked sialic acids for Sialidase A.