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Volume 32, Number 5—May 2026

Synopsis

Three Fatal Gestational Psittacosis Cases Caused by Chlamydia psittaci Strains Belonging to Closely Related Lineages, Japan

Atsuko Nishino, Yukiko Nakura, Yukiko Sassa-O’Brien, Momoko Soeda, Hirokazu Sugii, Kanako Shimizu, Shiro Miura, Yumiko Sato, Michinobu Yoshimura1, Michiko Kodama, and Itaru YanagiharaComments to Author 
Author affiliation: Research Institute, Osaka Women’s and Children’s Hospital, Osaka, Japan (A. Nishino, Y. Nakura, M. Yoshimura, I. Yanagihara); The University of Osaka, Osaka (A. Nishino, M. Kodama, I. Yanagihara); Tokyo University of Agriculture and Technology, Tokyo, Japan (Y. Sassa-O’Brien); NHO Nagasaki Medical Center, Nagasaki, Japan (M. Soeda, S. Miura); NHO Iwakuni Clinical Center, Yamaguchi, Japan (H. Sugii, Y. Sato); Tannan Health Welfare Center, Fukui, Japan (K. Shimizu); Maizuru Kyosai Hospital, Kyoto, Japan (K. Shimizu)

Main Article

Figure 4

Relative quantification of chlamydial DNA from the maternal lungs, placenta, and spleen in study of 3 fatal gestational psittacosis cases caused by C. psittaci strains belonging to closely related multilocus sequence typing lineages, Japan, 2017–2024. Quantitative real-time PCR was performed targeting the 16S rRNA gene of the order Chlamydiales (n = 3). Relative quantification was performed using the 2−ΔΔCt method, normalizing chlamydial DNA levels to β actin as internal control and calculating the relative fold change in the placenta and spleen compared with the lungs. A) Case FO-01; B) case YO-02; C) case NO-03.

Figure 4. Relative quantification of chlamydial DNA from the maternal lungs, placenta, and spleen in study of 3 fatal gestational psittacosis cases caused by C. psittaci strains belonging to closely related multilocus sequence typing lineages, Japan, 2017–2024. Quantitative real-time PCR was performed targeting the 16S rRNA gene of the order Chlamydiales (n = 3). Relative quantification was performed using the 2−ΔΔCt method, normalizing chlamydial DNA levels to β actin as internal control and calculating the relative fold change in the placenta and spleen compared with the lungs. A) Case FO-01; B) case YO-02; C) case NO-03.

Main Article

1Current affiliation: Fukuoka University, Fukuoka, Japan.

Page created: April 01, 2026
Page updated: May 08, 2026
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