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Volume 7, Number 3—June 2001

PulseNet: The Molecular Subtyping Network for Foodborne Bacterial Disease Surveillance, United States

Bala SwaminathanComments to Author , Timothy J. Barrett, Susan B. Hunter, Robert V. Tauxe, and the CDC PulseNet Task Force
Author affiliations: Centers for Disease Control and Prevention, Atlanta, Georgia USA

Main Article

Table 1

Interlaboratory reproducibility of pulsed-field gel electrophoresis patterns of 64 Escherichia coli isolates by eight laboratories following the PulseNet standardized protocol

Laboratory (%)
Result 1 2 3 4 5 6 7 8
Matched expected pattern 64/64a 63/63 59/63 62/64 62/64 62/64 61/64 61/64
(100) (100) (93.7) (96.9) (96.9) (96.9) (95.3) (95.3)
< 1-band difference from expected pattern 64/64 63/63 62/63 64/64 63/64 64/64 64/64 64/64
(100) (100) (98.4) (100) (98.4) (100) (100) (100)

aValues represent no. of patterns fitting in the specified category/no. of isolates tested.

Main Article

1The CDC PulseNet Task Force includes John Allan, Michelle Bird, Daniel Cameron, Wallis DeWitt, Thomas Donkar, Collette Fitzgerald, Judy Gaither, Lewis Graves, Peggy Hayes, Jared Kerr, Kristy Kubota, Mary Ann Lambert-Fair, Susan Maslanka, Loretta McCroskey, Jeremy Miller, Heather Noll, Efrain Ribot, Nicole Tucker, Susan Van Duyne, and the authors.

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