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Volume 15, Number 2—February 2009
Research

Simian T-Lymphotropic Virus Diversity among Nonhuman Primates, Cameroon

David M. Sintasath, Nathan D. Wolfe1, Matthew LeBreton, Hongwei Jia, Albert D. Garcia, Joseph Le Doux Diffo, Ubald Tamoufe, Jean K. Carr, Thomas M. Folks, Eitel Mpoudi-Ngole, Donald S. Burke2, Walid Heneine, and William M. SwitzerComments to Author 
Author affiliations: Johns Hopkins Bloomberg School of Public Health, Baltimore, Maryland, USA (D.M. Sintasath, D.S. Burke); University of California School of Public Health, Los Angeles, California, USA (N.D. Wolfe, U. Tamoufe); Johns Hopkins Cameroon Program, Yaoundé, Cameroon (M. LeBreton, J.L.D. Diffo, U. Tamoufe, E. Mpoudi-Ngole); Centers for Disease Control and Prevention, Atlanta, Georgia, USA (H. Jia, A. D. Garcia, T.M. Folks, W. Heneine, W.M. Switzer); University of Maryland Biotechnology Institute, Baltimore (J.K. Carr)

Main Article

Table 1

Nucleotide sequences of primer sets used to amplify tax and long terminal repeat sequences of simian T-lymphotropic virus–3 (Cmo8699AB and Cni7867AB)*

Region Primer set Forward primer and sequence (5′ → 3′) Reverse primer and sequence (5′ → 3′) bp
tax
 Outer 8699TF1
GTACCCTGTCTACGTTTTCGGCGAT PGTAXR1
GAIGAYTGIASTACYAAAGATGGCTG 779
Inner 8699TF2
TTACTGGCCACCTGTCCTGAACAC PGTAXR2
TTIGGGYAIGGICCGGAAATCAT 658
Outer P5TAXF3†
CCCTCAAGGTCCTCACCCCGCCGC P5TAXR3
TAACGGCCAGGTCATTGGAGGTGT 244
Inner
 P5TAXF2†
AAGTTCCTCCCTCCTTCTTCCATG
 P5TAXR1
TGGTAGAGGTATAAGCACACGATGGTG
 174
tax-LTR
 Outer 8699TF6
CATCCGGACCAACTAGGGGCCTTC PGTATA1+2R1
TCCTGAACYGTCYYYRCGCTTTTATAG 721
Inner 8699TF7‡
AACAAAAATCCCTACCAAACGCTT PGTATA1+2R1
TCCTGAACYGTCYYYRCGCTTTTATAG 695
Inner
 8699TF8§
CAGCCCACCCGCGCACCAGTAATT
 PGTATA1+2R1
TCCTGAACYGTCYYYRCGCTTTTATAG
 589
LTR Outer 8699LF3
CTCTGACGTCTCTCCCTGCCTTGT PGPBSR1n
ATCCCGGACGAGCCCCCA 612
Inner 8699LF4
CCGGAAAAAACCTTAAACCACCCA PGPBSR1n
ATCCCGGACGAGCCCCCA 585

*bp, base pair; LTR, long terminal repeat; I, inosine; S, G/C; Y, T/C; R, A/G. Nonhuman primates are coded as follows: the first letter of the genus is followed by the first 2 letters of the species name: Cmo, Cercopithecus mona; Cni, C. nictitans. The last 2 letters in the code indicate the study site: AB, Abat.
†Primers used to screen human peripheral blood mononuclear cell DNA for simian T-lymphotropic virus-3 (Cmo8699AB)–like tax sequences.
‡Primer set used for Cni7867AB.
§Primer set used for Cmo8699AB.

Main Article

1Current affiliation: Stanford University, Stanford, California, USA.

2Current affiliation: University of Pittsburgh Graduate School of Public Health, Pittsburgh, Pennsylvania, USA.

Page created: December 08, 2010
Page updated: December 08, 2010
Page reviewed: December 08, 2010
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