Volume 10, Number 9—September 2004
Research
Genotyping, Orientalis-like Yersinia pestis, and Plague Pandemics
Michel Drancourt*, Véronique Roux*, La Vu Dang*, Lam Tran-Hung*, Dominique Castex†, Viviane Chenal-Francisque‡, Hiroyuki Ogata§, Pierre-Edouard Fournier*, Eric Crubézy¶, and Didier Raoult*
Table 2
Location of primers used for PCR amplification and sequencing of eight intergenic spacers in Yersinia pestisa
| Spacer | Upstream gene (N) Downstream gene (N) | Primer sequence (5´ → 3´) |
|---|---|---|
| YP1 | ace K (YP03724) ace A (YP03725) | AATCCCTGCAAAATGGTCTG CTGATGGGAAGCAAAGGTGT |
| YP3 | glg P (YP03938) glg A (YP03939) | TCAGTGCATCCACACTGACA CGTATCGCCTTCACTAAGGC |
| YP4 | gene ID 1176814 (YP03976) gor (YP03977) | TAATCCGCCGTGGAAATTAG ACGATTATCTGGCAATTGGC |
| YP5 | Gene ID 1175557 (YP02727) Gene ID 1175558 (YP02728) | GCATGCGCTGTTTGATATTG TTATGACTCACGGACGATGC |
| YP7 | lex A (YP00314) Gene ID 1173160 (YP00315) | GTAACGGGGACTGGATCTGA ATAAACCGTGTGCTTCCACC |
| YP8 | Gene ID 1175559 (YP02729) Gene ID 1175560 (YP02730) | ACGGAAATTGCCAGATTCA GACTTGAGCTTCATTTGGCC |
| YP9 | mrd F (YP02648) mrd E (YP02649) | GCGCTGATACGTGTTATTGG TTGTTAATATCGCGGGTGGTA |
| YP10 | bio D (YP02269) Gene ID 1175101 (YP02270) | ATGCTGAAACAATCGCAATG CAATAAGGTGTACTCGCCGG |
aNumbering according to the Y. pestis CO92 strain genome, GenBank accession no. NC-223143). PCR, polymerase chain reaction.
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