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Volume 18, Number 1—January 2012


Assessing Prion Infectivity of Human Urine in Sporadic Creutzfeldt-Jakob Disease

Silvio Notari1, Liuting Qing1, Maurizio Pocchiari, Ayuna Dagdanova, Kristin Hatcher, Arend Dogterom, Jose F. Groisman, Ib Bo Lumholtz, Maria Puopolo, Corinne Lasmezas, Shu G. Chen, Qingzhong Kong, and Pierluigi GambettiComments to Author 
Author affiliations: Case Western Reserve University, Cleveland, Ohio, USA (S. Notari, L. Qing, A. Dagdanova, K. Hatcher, S.G. Chen, Q. Kong, P. Gambetti); Istituto Superiore di Sanità, Rome, Italy (M. Pocchiari, M. Puopolo); Ferring Pharmaceuticals, Hvidore, Denmark (A. Dogterom); Instituto Massone, Buenos Aires, Argentina (J.F. Groisman); BL Consult ApS, Copenhagen, Denmark (I.B. Lumholtz); The Scripps Research Institute, Jupiter, Florida, USA (C. Lasmezas)

Main Article

Table 3

Determination of infectivity loss of MF from sCJDMM1 patient 3 spiked in normal urine, during concentration and dialysis procedures*

Inoculum Brain† dilution Incubation time, d, mean ± SEM Distribution Prion titer, ID50/g tissue†
MF in normal urine, then concentrated 100× and dialyzed 10−2 265 ± 12‡ 8/9 8.2 × 106
MF in 100× concentrated and dialyzed normal human urine 10−2 268 ± 10‡ 8/8 6.9 × 106

*MF, microsomal fraction; sCJD, sporadic Creutzfeldt-Jakob disease; ID50, 50% infectious dose.
†Brain tissue equivalent.
‡p = 0.42 by t-test.

Main Article

1These authors contributed equally to this article.