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Volume 18, Number 1—January 2012


Assessing Prion Infectivity of Human Urine in Sporadic Creutzfeldt-Jakob Disease

Silvio Notari1, Liuting Qing1, Maurizio Pocchiari, Ayuna Dagdanova, Kristin Hatcher, Arend Dogterom, Jose F. Groisman, Ib Bo Lumholtz, Maria Puopolo, Corinne Lasmezas, Shu G. Chen, Qingzhong Kong, and Pierluigi GambettiComments to Author 
Author affiliations: Case Western Reserve University, Cleveland, Ohio, USA (S. Notari, L. Qing, A. Dagdanova, K. Hatcher, S.G. Chen, Q. Kong, P. Gambetti); Istituto Superiore di Sanità, Rome, Italy (M. Pocchiari, M. Puopolo); Ferring Pharmaceuticals, Hvidore, Denmark (A. Dogterom); Instituto Massone, Buenos Aires, Argentina (J.F. Groisman); BL Consult ApS, Copenhagen, Denmark (I.B. Lumholtz); The Scripps Research Institute, Jupiter, Florida, USA (C. Lasmezas)

Main Article

Table 2

Prion infectivity in microsomal fraction prepared from sCJDMM1 patient 4 and suspended in PBS or 100× concentrated and dialyzed urine*

Carrier Brain† dilution Incubation time, d, mean ± SEM Distribution Prion titer, ID50/g tissue†
PBS‡ 10−2 267 ± 14 5/5 7.2 × 106
100× concentrated and dialyzed normal human urine 10−1 262 ± 4 5/5 3.3 × 105
10−2 281 ± 7 5/5
10−3 377 ± 35 6/6
10−4 311, 335 2/4
10−5 >793 0/6
10−6 >702 0/6

*sCJD, sporadic Creutzfeldt-Jakob disease; PBS, phosphate-buffered saline; ID50, 50% infectious dose.
†Brain tissue equivalent.
‡Titer was the same as calculated in Table 1.

Main Article

1These authors contributed equally to this article.