Figure 6. . PrPres typing of the CWD amplification product. The CWD PMCA product derived from amplification in a human brain homogenate substrate (PRNP codon 129MM) was compared by Western blotting with PrPres from human brain samples from cases of sCJD of the MM1 subtype, sCJD of the VV2 subtype, and variant CJD. The PrP detection antibody was 3F4. PrPres, protease-resistant prion protein; CWD, chronic wasting disease; PMCA, protein misfolding cyclic amplification; sCJD sporadic Creutzfeldt-Jakob disease; vCJD, variant CJD; hu, human; M, molecular marker.