Figure 2. . PMCA of PRNP codon 129MM human brain homogenate and humanized transgenic mice brain homogenate seeded with C-BSE, scrapie, CWD, L-BSE, H-BSE, vCJD, and atypical scrapie. PMCA reactions using PRNP 129MM human brain homogenate (human postmortem tissue) (A) and PRNP 129MM humanized transgenic mouse brain homogenate (Tg-Hu) (B) were seeded (1:3) with animal prion disease brain as indicated. Lanes 1, 3, 5, 7, 9, 11, 13, and 15 show the samples without PMCA. Samples in lanes 2, 4, 6, 8, 10, 12, and 14 were subjected to PMCA. Western blotting used the antibody 3F4 that enables the specific detection of human PrP. To compare the PrPres levels into the seeds (before the PMCA), antibody 6H4 was also used. PMCA, protein misfolding cyclic amplification; BSE, bovine spongiform encephalopathy; CWD, chronic wasting disease; vCJD, variant Creutzfeldt-Jakob disease; PrP, prion protein; PrPres, protease-resistant PrP; M, molecular marker.