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Volume 20, Number 1—January 2014

Research

Molecular Barriers to Zoonotic Transmission of Prions

Marcelo A. Barria, Aru Balachandran, Masanori Morita, Tetsuyuki Kitamoto, Rona Barron, Jean Manson, Richard Knight, James W. Ironside, and Mark W. HeadComments to Author 
Author affiliations: The University of Edinburgh, Edinburgh, Scotland, UK (M.A. Barria, R. Knight, J.W. Ironside, M.W Head); Canadian Food Inspection Agency, Ottawa, Ontario, Canada (A. Balachandran); Japan Blood Products Organization, Kobe, Japan (M. Morita); Tohoku University Graduate School of Medicine, Sendai, Japan (T. Kitamoto); University of Edinburgh, Easter Bush, Scotland, UK (R. Barron, J. Manson)

Main Article

Table

Comparison of the outcomes of experimental transmission and in vitro conversion studies of chronic wasting disease in human, humanized, and nonhuman primate model systems*

Donor animal inoculum In vivo
In vitro
Ref
Species inoculated Animal (expression levels)/codon 129 Transmission PrP source (expression levels)/codon 129 Method Conversion
Mule deer, white-tailed deer, and elk


Cells 129M and 129V
C-FA
Pos
(28,29)
Elk
Humanized PrP transgenic mice
Tg-40 (1×)/129MM Neg

(24)
Tg-1 (2×)/129MM
Neg
Mule deer
Squirrel monkeys
Saimiri sciureus (1×)
Pos


(20)
Mule deer, white-tailed deer, and elk
Humanized PrP transgenic mice
Tg(HuPrP)440 (2×)
Neg


(27)
Mule deer, white-tailed deer, and elk
Squirrel monkeys Saimiri sciureus (1×) Pos

(22)
Cynomolgus macaques
Macaca fascicularis (1×)
Neg
Mule deer and white-tailed deer

Tg-6816 (16×)/129M PMCA Neg (31)

Tg-7823 (5×)/129V
PMCA
Neg
Mule deer
Humanized PrP transgenic mice
Tg-45 (4×)/129MM Neg
(26)
Tg-35 (2×)/129M Neg
Tg-152 (6×)/129VV
Neg

White-tailed deer
Humanized PrP transgenic mice
HuMM (1×)/129MM Neg

(11)
HuVV (1×)/129VV
Neg
Mule deer


Tg-440 (2×)/129MM
PMCA
Pos
(after in vitro conditioning)
(33)
Elk Human brain (1×)/129MM PMCA Pos This article
Human brain (1×)/129VV PMCA Pos
HuMM (1×)/129MM PMCA Pos
HuVV(1×)/129VV PMCA Pos
293F cell line(4×)/129M PMCA Pos
293F cell line(2×)/129V PMCA Pos

*Numbers in parentheses denote the stated expression levels of PrPc into the animal species and cell lines used. PrP, prion protein; C-FA, cell-free assay; pos, positive; neg, negative; PrPc, host’s normal cellular PrP. Blank cells indicate no reported data.

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