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Volume 20, Number 10—October 2014

Research

Clinical Isolates of Shiga Toxin 1a–Producing Shigella flexneri with an Epidemiological Link to Recent Travel to Hispañiola

Miranda D. Gray, Keith A. Lampel, Nancy A. Strockbine, Reinaldo E. Fernandez, Angela R. Melton-Celsa, and Anthony T. MaurelliComments to Author 
Author affiliations: Uniformed Services University of the Health Sciences, Bethesda, Maryland, USA (M.D. Gray, R.E. Fernandez, A.R. Melton-Celsa, A.T. Maurelli); US Food and Drug Administration, College Park, Maryland, USA (K.A. Lampel); Centers for Disease Control and Prevention, Atlanta, Georgia, USA (N.A. Strockbine)

Main Article

Figure 3

Infectious phage in the supernatants of Shiga toxin 1a gene (stx1a)–encoding Shigella flexneri are induced with mitomycin C (mito C) treatment. Supernatants were collected from exponential cultures of BS937, BS938, and BS974 grown with or without 0.5 μg/mL mito C for 3 h. The number of infectious phage particles was determined by a soft agar overlay method that used Escherichia coli MG1655 as the recipient. Plaque forming units (PFUs) of phage lysate were counted after 24 h incubationData are av

Figure 3. Infectious phage in the supernatants of Shiga toxin 1a gene (stx1a)–encoding Shigella flexneri are induced with mitomycin C (mito C) treatment. Supernatants were collected from exponential cultures of BS937, BS938, and BS974 grown with or without 0.5 μg/mL mito C for 3 h. The number of infectious phage particles was determined by a soft agar overlay method that used Escherichia coli MG1655 as the recipient. Plaque forming units (PFUs) of phage lysate were counted after 24 h incubation. Data are averages of 3 independent experiments. Error bars indicate standard error.

Main Article

Page created: September 12, 2014
Page updated: September 12, 2014
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