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Volume 20, Number 10—October 2014
Research

Clinical Isolates of Shiga Toxin 1a–Producing Shigella flexneri with an Epidemiological Link to Recent Travel to Hispañiola

Miranda D. Gray, Keith A. Lampel, Nancy A. Strockbine, Reinaldo E. Fernandez, Angela R. Melton-Celsa, and Anthony T. MaurelliComments to Author 
Author affiliations: Uniformed Services University of the Health Sciences, Bethesda, Maryland, USA (M.D. Gray, R.E. Fernandez, A.R. Melton-Celsa, A.T. Maurelli); US Food and Drug Administration, College Park, Maryland, USA (K.A. Lampel); Centers for Disease Control and Prevention, Atlanta, Georgia, USA (N.A. Strockbine)

Main Article

Figure 4

Schematic of PCR designed to determine that Shiga toxin 1a gene (stx1a) is phage encoded and inserted into the S1742 locus of Shigella flexneri. The genetic map shows the insertion of ϕPOC-J13 into locus S1742 of stx1a-encoding S. flexneri. Location and direction of the primers used for PCR analyses are indicated. The top part of the figure indicates the genes flanking the stx1a operon. Q, antiterminator; A and B, Stx1a subunits that form the assembled toxin (A is the stx1aA subunit and B is the

Figure 4. Schematic of PCR designed to determine that Shiga toxin 1a gene (stx1a) is phage encoded and inserted into the S1742 locus of Shigella flexneri. The genetic map shows the insertion of ϕPOC-J13 into locus S1742 of stx1a-encoding S. flexneri. Location and direction of the primers used for PCR analyses are indicated. The top part of the figure indicates the genes flanking the stx1a operon. Q, antiterminator; A and B, Stx1a subunits that form the assembled toxin (A is the stx1aA subunit and B is the stx1aB subunit); yshS, a hypothetical protein that shares homology with yjhS from Escherichia coli K-12; orf, open reading frame; S, gene that encodes the phage holin; R, gene that encodes the phage endolysin.

Main Article

Page created: September 12, 2014
Page updated: September 12, 2014
Page reviewed: September 12, 2014
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