Volume 20, Number 10—October 2014
Research
Clinical Isolates of Shiga Toxin 1a–Producing Shigella flexneri with an Epidemiological Link to Recent Travel to Hispañiola
Miranda D. Gray, Keith A. Lampel, Nancy A. Strockbine, Reinaldo E. Fernandez, Angela R. Melton-Celsa, and Anthony T. Maurelli
Table 2
Primer pairs used for PCR analysis
| Primer pair | Sequence, 5′ → 3′ | Amplicon size, bp | Source |
|---|---|---|---|
| stx1-det-F1 | GTACGGGGATGCAGATAAATCGC | 698 | (14) |
| stx1-seq-R1 | GAAGAAGAGACTGAAGATTCCATCTG | ||
| stx2_F4 | GGCACTGTCTGAAACTGCTCCTGT | 627 | (14) |
| stx2R1 | ATTAAACTGCACTTCAGCAAATCC | ||
| VirF1 | GCAAATACTTAGCTTGTTGCACAGAG | 907 | This study |
| VirF2 | GGGCTTGATATTCCGATAAGTC | ||
| VirB01 | TTCTACCATCAATCTCCCTTCC | 897 | This study |
| IpaAFwd | GTATCTAGCGCCCTCAGCAAG | ||
| IpaHF | GCGTTCCTTGACCGCCTTTCCGATACCG | 628 | This study |
| IpaHR | CTTTCAGCCGGTCAGCCACCCTCTGAGAG | ||
| Stx1R2 | AGCGAATGACATTCAGCGAATCTA | 1,059 | This study |
| Phage_stxR2 | GACGCCATACAAGGAGTC | ||
| Stx1F2 | ACGCCTGATTGTGTAACTGGAAA | 1,333 | This study |
| Phage_stx1F2 | CACTCGCGTCACTGTATG | ||
| Stx_phage_up | GACCGCACACTGTGCTATC | 1,155 | This study |
| S1742 up | CCGTGCGGGTATTTAACAATAATGG | ||
| Stx_phage_dn | AGTCAAACCGCGCTATTGG | 1,224 | This study |
| S1742 dn | TGCATGACAGAGGCAATAAACCCGAT | ||
| RecAko-site1 | GCTATCGACGAAAACAAACAGAAAGCGTTGGCGGCAGCACTGGGCCAGATTGTGTAGGCTGGAGCTGCTTC | 1,609 | This study |
| RecAko-site2 | AAAATCTTCGTTAGTTTCTGCTACTCCTTCGCTGTCATCTACAGAGAAATCCATATGAATATCCTCCTTA | ||
| RecA-1 | ACATATTGACTATCCGGTATTACCCGG | 1,148, 1,701* | This study |
| RecA-3 | GACCGTCCGTGCACACATTATCTATT |
*Amplicon sizes for wild-type recA or insertion of kan cassette into recA, respectively.
Page created: September 12, 2014
Page updated: September 12, 2014
Page reviewed: September 12, 2014
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