Volume 20, Number 10—October 2014
Research
Clinical Isolates of Shiga Toxin 1a–Producing Shigella flexneri with an Epidemiological Link to Recent Travel to Hispañiola
Table 2
Primer pairs used for PCR analysis
Primer pair | Sequence, 5′ → 3′ | Amplicon size, bp | Source |
---|---|---|---|
stx1-det-F1 | GTACGGGGATGCAGATAAATCGC | 698 | (14) |
stx1-seq-R1 | GAAGAAGAGACTGAAGATTCCATCTG | ||
stx2_F4 | GGCACTGTCTGAAACTGCTCCTGT | 627 | (14) |
stx2R1 | ATTAAACTGCACTTCAGCAAATCC | ||
VirF1 | GCAAATACTTAGCTTGTTGCACAGAG | 907 | This study |
VirF2 | GGGCTTGATATTCCGATAAGTC | ||
VirB01 | TTCTACCATCAATCTCCCTTCC | 897 | This study |
IpaAFwd | GTATCTAGCGCCCTCAGCAAG | ||
IpaHF | GCGTTCCTTGACCGCCTTTCCGATACCG | 628 | This study |
IpaHR | CTTTCAGCCGGTCAGCCACCCTCTGAGAG | ||
Stx1R2 | AGCGAATGACATTCAGCGAATCTA | 1,059 | This study |
Phage_stxR2 | GACGCCATACAAGGAGTC | ||
Stx1F2 | ACGCCTGATTGTGTAACTGGAAA | 1,333 | This study |
Phage_stx1F2 | CACTCGCGTCACTGTATG | ||
Stx_phage_up | GACCGCACACTGTGCTATC | 1,155 | This study |
S1742 up | CCGTGCGGGTATTTAACAATAATGG | ||
Stx_phage_dn | AGTCAAACCGCGCTATTGG | 1,224 | This study |
S1742 dn | TGCATGACAGAGGCAATAAACCCGAT | ||
RecAko-site1 | GCTATCGACGAAAACAAACAGAAAGCGTTGGCGGCAGCACTGGGCCAGATTGTGTAGGCTGGAGCTGCTTC | 1,609 | This study |
RecAko-site2 | AAAATCTTCGTTAGTTTCTGCTACTCCTTCGCTGTCATCTACAGAGAAATCCATATGAATATCCTCCTTA | ||
RecA-1 | ACATATTGACTATCCGGTATTACCCGG | 1,148, 1,701* | This study |
RecA-3 | GACCGTCCGTGCACACATTATCTATT |
*Amplicon sizes for wild-type recA or insertion of kan cassette into recA, respectively.