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Volume 20, Number 12—December 2014
Research

Replication and Shedding of MERS-CoV in Upper Respiratory Tract of Inoculated Dromedary Camels

Danielle R. Adney, Neeltje van Doremalen, Vienna R. Brown, Trenton Bushmaker, Dana Scott, Emmie de Wit, Richard A. Bowen1Comments to Author , and Vincent J. Munster1
Author affiliations: Colorado State University, Fort Collins, Colorado, USA; (D.R. Adney, V.R. Brown, R.A. Bowen); National Institutes of Health, Hamilton, Montana, USA (N. van Doremalen, T. Bushmaker, D. Scott, E. de Wit, V.J. Munster)

Main Article

Figure 3

Virus shedding determined by oral swabs obtained from dromedary camels inoculated with Middle East respiratory syndrome coronavirus (MERS-CoV). Shedding was determined by A) determining infectious titers and B) viral RNA. Virus titers were determined by plaque assay and viral load by qRT-PCR. TCID50 equivalents were extrapolated from standard curves generated by 10-fold dilutions of a MERS-CoV stock (HCoV-EMC/2012) with known virus titer in parallel to each run.

Figure 3. Virus shedding determined by oral swabs obtained from dromedary camels inoculated with Middle East respiratory syndrome coronavirus (MERS-CoV). Shedding was determined by A) determining infectious titers and B) viral RNA. Virus titers were determined by plaque assay and viral load by qRT-PCR. TCID50 equivalents were extrapolated from standard curves generated by 10-fold dilutions of a MERS-CoV stock (HCoV-EMC/2012) with known virus titer in parallel to each run.

Main Article

1These senior authors contributed equally to this article.

Page created: November 18, 2014
Page updated: November 18, 2014
Page reviewed: November 18, 2014
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