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Volume 21, Number 3—March 2015
Research

Nanomicroarray and Multiplex Next-Generation Sequencing for Simultaneous Identification and Characterization of Influenza Viruses

Jiangqin ZhaoComments to Author , Viswanath Ragupathy, Jikun Liu, Xue Wang, Sai Vikram Vemula, Haja Sittana El Mubarak, Zhiping Ye, Marie L. Landry, and Indira HewlettComments to Author 
Author affiliations: Food and Drug Administration, Silver Spring, Maryland, USA (J. Zhao, V. Ragupathy, J. Liu, X. Wang, S.V. Vemula, H.S. El Mubarak, Z. Ye, I. Hewlett); Yale University School of Medicine, New Haven, Connecticut, USA (M.L. Landry)

Main Article

Table 1

Detection of influenza A viruses in nasopharyngeal swab samples collected from naturally infected patients, Connecticut, USA, 2012–13 influenza season*

Patient ID Patient age, y/sex Sample collection
 Detection methods
Date, 2012 Location DFA rRT-PCR, Ct Universal PCR NGS
FLU001 47/F Dec 30 Hamden, CT + ND + H3N2
FLU002 80/M Dec 30 Milford, CT + 17.5 + H3N2
FLU004 35/F Dec 30 Meriden, CT + ND + H3N2
FLU006 25/M Dec 29 New Haven, CT + ND + H3N2
FLU007 23/F Dec 30 New Haven, CT + ND + H3N2
FLU008 31/F Dec 30 Trumbull, CT + 28.1 + H3N2
FLU009 68/M Dec 30 Hamden, CT + ND + H3N2
FLU012 35/F Dec 30 Rutledge, MO + ND + H3N2
FLU013 92/M Dec 29 Woodbridge, CT + 16.6 + H3N2
FLU014 84/F Dec 30 Chester, CT + 15.5 + H3N2
FLU017 66/F Dec 29 Clinton, CT + 24.3 + H3N2
FLU018 17/M Dec 31 New Haven, CT + 19.9 + H3N2
FLU021 63/F Dec 30 New Haven, CT + ND + H3N2
FLU023 55/F Dec 28 North Haven, CT + 21.8 + H3N2
FLU025 47/M Dec 30 West Haven, CT + ND + H3N2
FLU026 32/F Dec 28 West Haven, CT + ND + H3N2
FLU027 26/F Dec 30 Bridgeport, CT + ND + H3N2
FLU028 89/F Dec 29 Woodbridge, CT I 21.0 + H3N2
FLU033 82/F Dec 29 Guilford, CT + 19.8 + H3N2
FLU034 37/F Dec 27 New Haven, CT + ND + H3N2
FLU036 18/M Dec 29 New Haven, CT I 17.8 + H3N2
FLU037 8/F Dec 29 New Haven, CT + ND + H3N2
FLU038 21/M Dec 27 West Haven, CT + ND + H3N2
FLU040 23/M Dec 27 West Haven, CT + ND + H3N2

*Ct, cycle threshold value; DFA, direct fluorescent antigen test; I, inadequate cells for DFA; ID, identification; ND, not done; NGS, next-generation sequencing; rRT-PCR, real-time RT-PCR.

Main Article

Page created: February 18, 2015
Page updated: February 18, 2015
Page reviewed: February 18, 2015
The conclusions, findings, and opinions expressed by authors contributing to this journal do not necessarily reflect the official position of the U.S. Department of Health and Human Services, the Public Health Service, the Centers for Disease Control and Prevention, or the authors' affiliated institutions. Use of trade names is for identification only and does not imply endorsement by any of the groups named above.
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