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Volume 21, Number 3—March 2015

Nanomicroarray and Multiplex Next-Generation Sequencing for Simultaneous Identification and Characterization of Influenza Viruses

Jiangqin ZhaoComments to Author , Viswanath Ragupathy, Jikun Liu, Xue Wang, Sai Vikram Vemula, Haja Sittana El Mubarak, Zhiping Ye, Marie L. Landry, and Indira HewlettComments to Author 
Author affiliations: Food and Drug Administration, Silver Spring, Maryland, USA (J. Zhao, V. Ragupathy, J. Liu, X. Wang, S.V. Vemula, H.S. El Mubarak, Z. Ye, I. Hewlett); Yale University School of Medicine, New Haven, Connecticut, USA (M.L. Landry)

Main Article

Table 1

Detection of influenza A viruses in nasopharyngeal swab samples collected from naturally infected patients, Connecticut, USA, 2012–13 influenza season*

Patient ID Patient age, y/sex Sample collection
Detection methods
Date, 2012 Location DFA rRT-PCR, Ct Universal PCR NGS
FLU001 47/F Dec 30 Hamden, CT + ND + H3N2
FLU002 80/M Dec 30 Milford, CT + 17.5 + H3N2
FLU004 35/F Dec 30 Meriden, CT + ND + H3N2
FLU006 25/M Dec 29 New Haven, CT + ND + H3N2
FLU007 23/F Dec 30 New Haven, CT + ND + H3N2
FLU008 31/F Dec 30 Trumbull, CT + 28.1 + H3N2
FLU009 68/M Dec 30 Hamden, CT + ND + H3N2
FLU012 35/F Dec 30 Rutledge, MO + ND + H3N2
FLU013 92/M Dec 29 Woodbridge, CT + 16.6 + H3N2
FLU014 84/F Dec 30 Chester, CT + 15.5 + H3N2
FLU017 66/F Dec 29 Clinton, CT + 24.3 + H3N2
FLU018 17/M Dec 31 New Haven, CT + 19.9 + H3N2
FLU021 63/F Dec 30 New Haven, CT + ND + H3N2
FLU023 55/F Dec 28 North Haven, CT + 21.8 + H3N2
FLU025 47/M Dec 30 West Haven, CT + ND + H3N2
FLU026 32/F Dec 28 West Haven, CT + ND + H3N2
FLU027 26/F Dec 30 Bridgeport, CT + ND + H3N2
FLU028 89/F Dec 29 Woodbridge, CT I 21.0 + H3N2
FLU033 82/F Dec 29 Guilford, CT + 19.8 + H3N2
FLU034 37/F Dec 27 New Haven, CT + ND + H3N2
FLU036 18/M Dec 29 New Haven, CT I 17.8 + H3N2
FLU037 8/F Dec 29 New Haven, CT + ND + H3N2
FLU038 21/M Dec 27 West Haven, CT + ND + H3N2
FLU040 23/M Dec 27 West Haven, CT + ND + H3N2

*Ct, cycle threshold value; DFA, direct fluorescent antigen test; I, inadequate cells for DFA; ID, identification; ND, not done; NGS, next-generation sequencing; rRT-PCR, real-time RT-PCR.

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Page created: February 18, 2015
Page updated: February 18, 2015
Page reviewed: February 18, 2015
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