Whole-Genome Characterization and Strain Comparison of VT2f-Producing Escherichia coli Causing Hemolytic Uremic Syndrome
Laura Grande
, Valeria Michelacci, Roslen Bondì, Federica Gigliucci, Eelco Franz, Mahdi Askari Badouei, Sabine Schlager, Fabio Minelli, Rosangela Tozzoli, Alfredo Caprioli, and Stefano Morabito
Author affiliations: Istituto Superiore di Sanità, Rome, Italy (L. Grande, V. Michelacci, R. Bondi, F. Gigliucci, F. Minelli, R. Tozzoli, A. Caprioli, S. Morabito); National Institute for Public Health and the Environment, Bilthoven, the Netherlands (E. Franz); Faculty of Veterinary Medicine, Garmsar Branch, Islamic Azad University, Garmsar, Iran (M. Askari Badouei); Austrian Agency for Health and Food Safety, Graz, Austria (S. Schlager)
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Figure 3
Figure 3. Long PCR analysis of the VT2f phage of verotoxigenic Escherichia coli (VTEC) strains isolated from fecal samples from humans with hemolytic uremic syndrome (EF467, EF476, EF453) and from pigeon feces (ED363, ED377). Numbers at left indicate bps; lane numbers indicate PCR 1 to PCR 4. The expected size of the amplicons were 6,331 bp (PCR 1), 8,166 bp (PCR 2), 3,927 bp (PCR 3), and 8,808 bp (PCR 4).
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