Volume 23, Number 12—December 2017
New Avian Hepadnavirus in Palaeognathous Bird, Germany
In 2015, we identified an avian hepatitis B virus associated with hepatitis in a group of captive elegant-crested tinamous (Eudromia elegans) in Germany. The full-length genome of this virus shares <76% sequence identity with other avihepadnaviruses. The virus may therefore be considered a new extant avian hepadnavirus.
Hepatitis B virus (HBV) belongs to the family Hepadnaviridae, members of which constitute 2 major extant genera: Orthohepadnavirus, which infect mammals, and Avihepadnavirus, which infect birds (1). Recently, evidence of a likely third genus was found with the discovery of a new fish hepadnavirus (2). In addition, HBV-derived endogenous viral elements have been reported in several neoavian birds (e.g., budgerigars and several finches) (3,4) and reptiles (e.g., turtles and crocodiles) (5).
Hepadnaviruses generally are characterized by their narrow host range and strong hepatotropism (1). They are enveloped, partially double-stranded DNA viruses with a small circular genome (≈3 kb) and at least 3 open reading frames (ORFs) (1). In orthohepadnaviruses, a fourth ORF encodes the X protein, which is associated with hepatocellular carcinoma in their respective host species. Avihepadnaviruses appear to have an X-like protein region; however, either a premature stop codon is present or no ORF is found in most cases (6). We describe a new avian HBV causing severe hepatitis in the elegant-crested tinamou (Eudromia elegans), a member of the ancient group of birds the Palaeognathae, which includes emus (Dromaius novaehollandiae) and ostriches (Struthio spp.).
In 2015, a deceased adult elegant-crested tinamou kept at Wuppertal Zoo (Wuppertal, Germany) underwent necropsy at the University of Veterinary Medicine Hannover, Foundation (Hannover, Germany). Initial histologic examination revealed moderate, necrotizing hepatitis and inclusion body–like structures within the hepatocytes. To identify a putative causative agent, we isolated nucleic acids from the liver and prepared them for sequencing on an Illumina MiSeq system (Illumina, San Diego, CA, USA) (Technical Appendix). We compared obtained reads with sequences in GenBank using an in-house metagenomics pipeline. Approximately 78% of the reads aligned to existing avihepadnavirus sequences. A full genome (3,024 bp) of the putative elegant-crested tinamou HBV (ETHBV) was subsequently constructed by de novo assembly mapping >2 million reads (88.6%) to the virus genome (GenBank accession no. KY977506).
The newly identified ETHBV shared <76% nt sequence identity with other avian HBVs (Technical Appendix Table 1). Phylogenetic analysis showed that ETHBV clustered within the genus Avihepadnavirus, forming a new clade (Figure, panel A). The organization of the ETHBV genome was similar to other avian HBVs because all 3 overlapping ORFs (polymerase, nucleocapsid [preC/C] and presurface [preS/S] antigen) and several essential sequence motifs (e.g., the epsilon element, TATA boxes, and direct repeat sites DR1 and DR2) were identified (Technical Appendix Figure 1). We also found an X-like sequence. However, similar to duck HBV, ETHBV lacks a putative translation start site. It has been suggested that the X protein evolved later in mammalian hosts (5), which explains the absence of X-like ORF in the ETHBV genome. Comparison of pairwise amino acid identities between ETHBV and other avihepadnaviruses showed low homologies between their functional proteins (64%–69% similarity to the polymerase, 75%–80% to the preC/C, 52%–62% to the preS/S [Technical Appendix Table 2]).
The identification of ETHBV prompted us to retrospectively screen the flock of 7 elegant-crested tinamous at Wuppertal Zoo and the 6 that had died within the past 4 years and had undergone necropsy at the University of Veterinary Medicine Hannover, Foundation (Technical Appendix Table 4). For that purpose, we designed a set of degenerated primers targeting a short region of the polymerase–preC/C genome in all avihepadnaviruses (Technical Appendix). All birds were found positive by PCR (Technical Appendix Table 4), including liver tissue from embryonated eggs, implying that ETHBV is vertically transmitted (Figure, panel B). We then obtained a second ETHBV genome (GenBank accession no. KY977507) from another tinamou from the same flock by deep sequencing; this genome showed 99.8% nt sequence identity with the initial ETHBV genome. Tinamou serum samples from another zoo were also screened but tested negative by PCR (Technical Appendix Table 4).
To further characterize ETHBV, we confirmed infection in the liver using an in situ hybridization protocol (8) in an adult and embryo tinamou (Figure, panel B). In addition to ETHBV infection in the liver, we found some positive cells in kidney and testis tissue. Although hepadnaviruses generally are host restricted, exceptions have been reported (e.g., crane HBV) (9). We attempted to infect Pekin duck embryos through the allantoic cavity, as well as by intravenous infection routes, and were not able to demonstrate replication (data not shown).
ETHBV can be considered a new extant hepadnavirus associated with hepatitis in the elegant-crested tinamou. Whether ETHBV can infect other species within the Palaeognathae or whether it is host restricted within other tinamou species remains to be elucidated. The discovery of ETHBV suggests that other avian species may harbor as-yet undiscovered HBVs. The pathogenesis of avian hepadnavirus infections and the mechanisms of virus transmission in captive tinamou flocks warrant further investigation.
Dr. Jo is a PhD candidate at the University of Veterinary Medicine Hannover Research Center for Emerging Infections and Zoonoses, Hannover, Germany. Her research interests include virus discovery, emerging and re-emerging infections, cross-species transmission, viral evolution, and host adaptation.
We thank Mareike Schubert, Kerstin Rohn, Danuta Waschke, Bettina Buck, Caroline Schütz, Kerstin Schöne, Heike Klippert-Hasberg, Mogens Drabert, Stefan Neander, and Jörn Wrede for excellent technical assistance. We also are grateful to Severin Dressen and Emile Prins for their collaboration in the project.
This study was in part supported by a grant from the Niedersachsen-Research Network on Neuroinfectiology from the Ministry of Science and Culture of Lower Saxony, Germany. This study also was in part supported by the COMPARE project and received funding from the European Union’s Horizon 2020 research and innovation program COMPARE (grant agreement no. 643476).
- Funk A, Mhamdi M, Will H, Sirma H. Avian hepatitis B viruses: molecular and cellular biology, phylogenesis, and host tropism. World J Gastroenterol. 2007;13:91–103. DOIPubMedGoogle Scholar
- Hahn CM, Iwanowicz LR, Cornman RS, Conway CM, Winton JR, Blazer VS. Characterization of a novel hepadnavirus in the white sucker (Catostomus commersonii) from the Great Lakes region of the United States. J Virol. 2015;89:11801–11. DOIPubMedGoogle Scholar
- Liu W, Pan S, Yang H, Bai W, Shen Z, Liu J, et al. The first full-length endogenous hepadnaviruses: identification and analysis. J Virol. 2012;86:9510–3. DOIPubMedGoogle Scholar
- Suh A, Brosius J, Schmitz J, Kriegs JO. The genome of a Mesozoic paleovirus reveals the evolution of hepatitis B viruses. Nat Commun. 2013;4:1791. DOIPubMedGoogle Scholar
- Suh A, Weber CC, Kehlmaier C, Braun EL, Green RE, Fritz U, et al. Early mesozoic coexistence of amniotes and hepadnaviridae. PLoS Genet. 2014;10:e1004559. DOIPubMedGoogle Scholar
- Chang SF, Netter HJ, Hildt E, Schuster R, Schaefer S, Hsu YC, et al. Duck hepatitis B virus expresses a regulatory HBx-like protein from a hidden open reading frame. J Virol. 2001;75:161–70. DOIPubMedGoogle Scholar
- Huelsenbeck JP, Ronquist F. MRBAYES: Bayesian inference of phylogenetic trees. Bioinformatics. 2001;17:754–5. DOIPubMedGoogle Scholar
- Pfankuche VM, Bodewes R, Hahn K, Puff C, Beineke A, Habierski A, et al. Porcine bocavirus infection associated with encephalomyelitis in a pig, Germany. Emerg Infect Dis. 2016;22:1310–2. DOIPubMedGoogle Scholar
- Prassolov A, Hohenberg H, Kalinina T, Schneider C, Cova L, Krone O, et al. New hepatitis B virus of cranes that has an unexpected broad host range. J Virol. 2003;77:1964–76. DOIPubMedGoogle Scholar
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1These authors contributed equally to this article.
Table of Contents – Volume 23, Number 12—December 2017
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Erhard van der Vries, Department of Infectious Diseases & Immunology, Faculty of Veterinary Medicine, Section Virology, Utrecht University, Utrecht, the Netherlands