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Volume 23, Number 12—December 2017


Spread of Canine Influenza A(H3N2) Virus, United States

Ian E.H. Voorhees, Amy L. Glaser, Kathy L. Toohey-Kurth, Sandra Newbury, Benjamin D. Dalziel, Edward Dubovi, Keith Poulsen, Christian Leutenegger, Katriina J.E. Willgert, Laura Brisbane-Cohen, Jill Richardson-Lopez, Edward C. Holmes, and Colin R. ParrishComments to Author 
Author affiliations: Cornell University, Ithaca, New York, USA (I.E.H. Voorhees, A.L. Glaser, E.J. Dubovi, K.J.E. Willgert, L. Brisbane-Cohen, C.R. Parrish); University of Wisconsin, Madison, Wisconsin, USA (K. Toohey-Kurth, S. Newbury, K. Poulsen); Oregon State University, Corvallis, Oregon, USA (B.D. Dalziel); IDEXX Laboratories, West Sacramento, California, USA (C. Leutenegger); Royal Veterinary College, London, UK (K.J.E. Willgert); Merck Animal Health, Madison, New Jersey, USA (J. Richardson-Lopez); University of Sydney, Sydney, New South Wales, Australia (E.C. Holmes)

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Table 1

Persistence of virus or viral RNA in dogs in 2 separate Chicago-area animal shelters that were infected with canine influenza A(H3N2) virus, United States, April 2015*

Shelter 2
1 Neg 37.4 25.1 37.9 26 29.5†
5 NT NT 22.7 24.6† NT NT
6 24.6† 23.7† NT NT NT NT
10 36.2 39.5 NT NT NT NT
11 NT NT NT NT 37.1 NT
15 NT NT 38.6† 35.9 38.2† 36
20 37.3 37.2 NT NT NT NT
23 NT NT Neg NT NT Neg
24 Neg Neg NT NT NT NT
28 Neg Neg NT NT NT NT

*Data from Newbury et al. (33) Table 1, used with permission. Data shown as rRT-PCR Ct values. Values <36 are considered positive results (black cells), and values >37 but <40 are considered weak positive results (dark gray cells). Light gray cells indicate negative rRT-PCR assay results for influenza A virus shedding. Ct, cycle threshold; Neg, negative; NT, not tested; rRT-PCR, real-time reverse transcription PCR.
†Samples for which virus was successfully isolated.

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