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Volume 23, Number 12—December 2017


Spread of Canine Influenza A(H3N2) Virus, United States

Ian E.H. Voorhees, Amy L. Glaser, Kathy L. Toohey-Kurth, Sandra Newbury, Benjamin D. Dalziel, Edward Dubovi, Keith Poulsen, Christian Leutenegger, Katriina J.E. Willgert, Laura Brisbane-Cohen, Jill Richardson-Lopez, Edward C. Holmes, and Colin R. ParrishComments to Author 
Author affiliations: Cornell University, Ithaca, New York, USA (I.E.H. Voorhees, A.L. Glaser, E.J. Dubovi, K.J.E. Willgert, L. Brisbane-Cohen, C.R. Parrish); University of Wisconsin, Madison, Wisconsin, USA (K. Toohey-Kurth, S. Newbury, K. Poulsen); Oregon State University, Corvallis, Oregon, USA (B.D. Dalziel); IDEXX Laboratories, West Sacramento, California, USA (C. Leutenegger); Royal Veterinary College, London, UK (K.J.E. Willgert); Merck Animal Health, Madison, New Jersey, USA (J. Richardson-Lopez); University of Sydney, Sydney, New South Wales, Australia (E.C. Holmes)

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Table 2

Results of the rRT-PCR analysis of a necropsied dog in a Chicago-area animal shelter that died after being infected with canine influenza A(H3N2) virus, United State, February 2015*

Type of specimen Ct value
Nasal swab 20.0
Oral swab 34.3
Tracheal swab 36.4
Bronchial swab 28.1
Cranial lung 31.8
Tracheobronchial lymph node 39.6
Liver Neg
Pancreas Neg
Ileum Neg
*Data from Watson et al. (32), used with permission. Data shown as rRT-PCR Ct values. Values <36 are considered positive results (black cells), and values >37 but <40 are considered weak positive results (dark gray cells). Light gray cells indicate negative rRT-PCR assay results for influenza A virus shedding. Ct, cycle threshold; Neg, negative; rRT-PCR, real-time reverse transcription PCR.

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