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Volume 23, Number 3—March 2017
Research

Whole-Genome Analysis of Bartonella ancashensis, a Novel Pathogen Causing Verruga Peruana, Rural Ancash Region, Peru

Kristin E. Mullins12Comments to Author , Jun Hang2, Robert J. Clifford2, Fatma Onmus-Leone, Yu Yang, Ju Jiang, Mariana Leguia, Matthew R. Kasper, Ciro Maguina, Emil P. Lesho, Richard G. Jarman, Allen L. Richards, and David Blazes
Author affiliations: Uniformed Services University of the Health Sciences, Bethesda, Maryland, USA (K.E. Mullins, J. Jiang, A. Richards, D. Blazes); US Naval Medical Research Center, Silver Spring, Maryland, USA (K.E. Mullins, A. Richards); Walter Reed Army Institute of Research, Silver Spring (J. Hang, R.J. Clifford, F. Onmus-Leone, Y. Yang, E.P. Lesho, R.G. Jarman); US Naval Medical Research Unit No. 6, Lima, Peru (M. Leguia, M.R. Kasper); Universidad Peruana Cayetano Heredia, Lima (C. Maguina)

Main Article

Table 1

Characteristics of 4 non-bacilliformis Bartonella isolates from 2 patients with verruga peruana, rural Ancash region, Peru*

Characteristic Patient 20, 3-y-old boy, isolate no.
 Patient 41, 10-y-old boy, isolate no.
20.00 20.60 41.00 41.60
Patient signs
 Lesions on hands and feet that disappeared after antimicrobial drug treatment
 Lesions on hands and feet that disappeared after antimicrobial drug treatment
Antimicrobial drugs used Azithromycin on days 0 and 7 Rifampin daily on days 0–14
Whole blood collection time
 Day 0
 Day 60
 Day 0
 Day 60
Peripheral blood smear† Negative Negative Negative Negative
Blood culture for Bartonella sp. Positive Positive Positive Positive
16S 321/533 TaqMan qPCR‡ Negative Negative 3.93 × 105 (19.24) 6.36 × 104 (22.15)
16S 27F2/533R PCR Negative Negative Positive Positive
B. ancashensis–specific PCR§
 Negative
 Negative
 Positive
 Positive
Blood culture gltA PCR/sequencing B. ancashensis B. ancashensis B. bacilliformis B. ancashensis
Isolate by pure-culture sequencing rrs, gltA, rpoB; whole genome rrs, gltA, rpoB; whole genome gltA rrs, gltA, rpoB; whole genome

*gltA, citrate synthase gene; rpoB, β subunit of RNA polymerase gene; rrs, 16S rRNA gene.
†Rapid microscopic diagnosis for Carrion’s disease (16).
‡Values are 16S rRNA gene copy number/microliter (quantitative cycle threshold).
§Primers 22RC-3F (5′-TTCGGCTTAGCTTATCCGTTTCACAA-3′) and 32RC-5R (5′-CGTAAGAGCTTTGTGGCAAAATAGCAA-3′) were used; the expected PCR amplicons size was 0.8 kb, which corresponds to nt 673839–674636 of B. ancashensis (GenBank accession no. CP010401).

Main Article

1Current affiliation: University of Maryland, Baltimore, Maryland, USA.

2These authors contributed equally to this article.

Page created: February 17, 2017
Page updated: February 17, 2017
Page reviewed: February 17, 2017
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