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Volume 23, Number 3—March 2017
Research

Zika Virus RNA Replication and Persistence in Brain and Placental Tissue

Julu BhatnagarComments to Author , Demi B. Rabeneck, Roosecelis B. Martines, Sarah Reagan-Steiner, Yokabed Ermias, Lindsey B.C. Estetter, Tadaki Suzuki, Jana M. Ritter, M. Kelly Keating, Gillian Hale, Joy Gary, Atis Muehlenbachs, Amy J. Lambert, Robert Lanciotti, Titilope Oduyebo, Dana Meaney-Delman, Fernando Bolaños, Edgar Alberto Parra Saad, Wun-Ju Shieh, and Sherif Zaki
Author affiliations: Centers for Disease Control and Prevention, Atlanta, Georgia, USA (J. Bhatnagar, D.B. Rabeneck, R.B. Martines, S. Reagan-Steiner, Y. Ermias, L.B.C. Estetter, T. Suzuki, J. Ritter, M.K. Keating, G. Hale, J. Gary, A. Muehlenbachs, T. Oduyebo, D. Meaney-Delman, W. Shieh, S.R. Zaki); Centers for Disease Control and Prevention, Fort Collins, Colorado, USA (A. Lambert, R. Lanciotti); Patología Hospital Universitario de Neiva, Neiva, Colombia (F. Bolaños); Instituto Nacional de Salud, Bogota, Colombia (E.A. Parra Saad)

Main Article

Figure 3

Localization of Zika virus RNA by ISH in placental tissues of women after spontaneous abortion. A) ISH with use of antisense probe. Zika virus genomic RNA localization in placental chorionic villi, predominantly within Hofbauer cells (red stain, arrows), of a case-patient who had spontaneous abortion at 11 wk gestation (case-patient no. 56). Original magnification ×10). B) ISH with use of sense probe. Serial section showing negative–strand replicative RNA intermediates (red stain, arrows) in the

Figure 3. Localization of Zika virus RNA by ISH in placental tissues of women after spontaneous abortion. A) ISH with use of antisense probe. Zika virus genomic RNA localization in placental chorionic villi, predominantly within Hofbauer cells (red stain, arrows), of a case-patient who had spontaneous abortion at 11 wk gestation (case-patient no. 56). Original magnification ×10). B) ISH with use of sense probe. Serial section showing negative–strand replicative RNA intermediates (red stain, arrows) in the same cells shown in panel A. Original magnification ×10. C) Hematoxylin and eosin stain of placental tissue of a case-patient who experienced spontaneous abortion at 8 wk gestation (case-patient no. 47). Original magnification ×20. D) Immunostaining for CD163 highlighting villous Hofbauer cells in a serial section as seen in panel C. Original magnification ×63. E) ISH with use of antisense probe. Zika virus genomic RNA as seen in a serial section from the same case-patient as in panel C, showing staining within Hofbauer cells (red stain, arrows) of placental chorionic villi. Original magnification ×40. F) ISH with use of sense probe. Serial section showing negative-strand replicative RNA intermediates (red stain, arrows) in the same cells as shown in panel E. Original magnification ×40. G) Hematoxylin and eosin stain from the same case-patient as in panel C, showing inflammatory cell infiltrates in maternal side of placenta. Original magnification ×63. H) ISH with use of sense probe. Negative-strand replicative RNA intermediates (red stain, arrows) in inflammatory cells in a serial section. Original magnification ×63. ISH, in situ hybridization.

Main Article

Page created: February 17, 2017
Page updated: February 17, 2017
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The conclusions, findings, and opinions expressed by authors contributing to this journal do not necessarily reflect the official position of the U.S. Department of Health and Human Services, the Public Health Service, the Centers for Disease Control and Prevention, or the authors' affiliated institutions. Use of trade names is for identification only and does not imply endorsement by any of the groups named above.
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