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Volume 23, Number 3—March 2017
Research

Zika Virus RNA Replication and Persistence in Brain and Placental Tissue

Julu BhatnagarComments to Author , Demi B. Rabeneck, Roosecelis B. Martines, Sarah Reagan-Steiner, Yokabed Ermias, Lindsey B.C. Estetter, Tadaki Suzuki, Jana M. Ritter, M. Kelly Keating, Gillian Hale, Joy Gary, Atis Muehlenbachs, Amy J. Lambert, Robert Lanciotti, Titilope Oduyebo, Dana Meaney-Delman, Fernando Bolaños, Edgar Alberto Parra Saad, Wun-Ju Shieh, and Sherif Zaki
Author affiliations: Centers for Disease Control and Prevention, Atlanta, Georgia, USA (J. Bhatnagar, D.B. Rabeneck, R.B. Martines, S. Reagan-Steiner, Y. Ermias, L.B.C. Estetter, T. Suzuki, J. Ritter, M.K. Keating, G. Hale, J. Gary, A. Muehlenbachs, T. Oduyebo, D. Meaney-Delman, W. Shieh, S.R. Zaki); Centers for Disease Control and Prevention, Fort Collins, Colorado, USA (A. Lambert, R. Lanciotti); Patología Hospital Universitario de Neiva, Neiva, Colombia (F. Bolaños); Instituto Nacional de Salud, Bogota, Colombia (E.A. Parra Saad)

Main Article

Table 1

Oligonucleotide primers used for RT-PCR assays*

RT-PCR Primers Sequence, 5′→3′ Gene target Product size, bp Annealing temperature, °C Reference
Zika virus
Forward AAG TAC ACA TAC CAA AAC AAA GTG GT NS5
127
56
This study
Reverse
TGT TAA GAG CGT AAG TGA CAA C
Zika virus
Forward TGC CCA ACA CAA GGT GAA GC E
209
58
This study
Reverse
ACT GAC AGC ATT ATC CGG TAC TC
DENV1–4
Forward AAG GAC TAG AGG TTA KAG GAG ACC C 3′ UTR
110
62
(27)
Reverse
GGC GYT CTG TGC CTG GAW TGA TG
CHIKV Forward TCA CTC CCT GTT GGA CTT GAT AGA PPG 126 55 (28)
Reverse TTG ACG AAC AGA GTT AGG AAC ATA CC

*CHIKV, chikungunya virus; DENV, dengue virus; E, envelope; NS, nonstructural; PPG, polyprotein gene; RT-PCR, reverse transcription PCR; UTR, untranslated region.

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