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Volume 23, Number 3—March 2017

Research

Zika Virus RNA Replication and Persistence in Brain and Placental Tissue

Julu BhatnagarComments to Author , Demi B. Rabeneck, Roosecelis B. Martines, Sarah Reagan-Steiner, Yokabed Ermias, Lindsey B.C. Estetter, Tadaki Suzuki, Jana M. Ritter, M. Kelly Keating, Gillian Hale, Joy Gary, Atis Muehlenbachs, Amy J. Lambert, Robert Lanciotti, Titilope Oduyebo, Dana Meaney-Delman, Fernando Bolaños, Edgar Alberto Parra Saad, Wun-Ju Shieh, and Sherif R. Zaki
Author affiliations: Centers for Disease Control and Prevention, Atlanta, Georgia, USA (J. Bhatnagar, D.B. Rabeneck, R.B. Martines, S. Reagan-Steiner, Y. Ermias, L.B.C. Estetter, T. Suzuki, J. Ritter, M.K. Keating, G. Hale, J. Gary, A. Muehlenbachs, T. Oduyebo, D. Meaney-Delman, W. Shieh, S.R. Zaki); Centers for Disease Control and Prevention, Fort Collins, Colorado, USA (A. Lambert, R. Lanciotti); Patología Hospital Universitario de Neiva, Neiva, Colombia (F. Bolaños); Instituto Nacional de Salud, Bogota, Colombia (E.A. Parra Saad)

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Table 4

Characteristics and laboratory findings of case-patients with adverse pregnancy outcome*

Case-patient no. Maternal travel history or residence Maternal symptom onset, gestation wk/trimester Outcome End of pregnancy, gestational age, wk/trimester Results of Zika virus testing performed on FFPE tissues
RT-PCR ISH†
81 Colombia 1/first SA 6/first Positive (placenta) Positive (placenta)
47 Honduras‡ 5/first SA ≈8/first Positive (placenta) Positive (placenta)
57 Puerto Rico‡ 5/first SA 8/firstt Positive (placenta) Positive (placenta)
56 Guatemala‡ 6/first SA 11/first Positive (placenta) Positive (placenta)
18 American Samoa 7/first SA 14/second Positive (placenta, fetal tissue) Positive (placenta, fetal tissue)
78 Colombia 7/first SA 11/first Positive (placenta) Positive (placenta)
125 Brazil 8/first SA 11/first Positive (placenta) Positive (placenta)
256 Brazil 8/first SA 13/first Positive (placenta) Negative (placenta)
79 Colombia NA/first SA 9/first Positive (placenta) Positive (placenta)
80 Mexico‡ 6/first SA 12/first Negative (placenta, cord) ND
19 Dominican Republic‡ 1/first SA 10/first Negative (placenta) ND
45 Honduras‡ 13/first ET 19/second Positive (placenta); negative umbilical cord, fetal brain, liver, lung) Positive (placenta)
76 Puerto Rico 7/first ET 9/first Positive (placenta) Negative (placenta)
28 Dominican Republic‡ NA/first ET 16/second Positive (placenta) Negative (placenta)
97 El Salvador NA/first IUFD§ 34/third Negative (placenta) ND
92 American Samoa NA/second IUFD 24/second Negative (placenta, umbilical cord, membrane) ND
556 Marshall Islands‡ 31/third IUFD 36/third Negative (placenta, umbilical cord, membrane) ND

*ET, elective termination; FFPE, formalin-fixed, paraffin-embedded; ISH, in situ hybridization; IUFD, intrauterine fetal demise; NA, not available; ND, not done; RT-PCR, reverse transcription PCR; SA, spontaneous abortion.
†ISH results include staining by both sense and antisense probes.
‡Travel history.
§With microcephaly.

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