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Volume 23, Number 3—March 2017

Research

Zika Virus RNA Replication and Persistence in Brain and Placental Tissue

Julu BhatnagarComments to Author , Demi B. Rabeneck, Roosecelis B. Martines, Sarah Reagan-Steiner, Yokabed Ermias, Lindsey B.C. Estetter, Tadaki Suzuki, Jana M. Ritter, M. Kelly Keating, Gillian Hale, Joy Gary, Atis Muehlenbachs, Amy Lambert, Robert Lanciotti, Titilope Oduyebo, Dana Meaney-Delman, Fernando Bolaños, Edgar Alberto Parra Saad, Wun-Ju Shieh, and Sherif R. Zaki
Author affiliations: Centers for Disease Control and Prevention, Atlanta, Georgia, USA (J. Bhatnagar, D.B. Rabeneck, R.B. Martines, S. Reagan-Steiner, Y. Ermias, L.B.C. Estetter, T. Suzuki, J. Ritter, M.K. Keating, G. Hale, J. Gary, A. Muehlenbachs, T. Oduyebo, D. Meaney-Delman, W. Shieh, S.R. Zaki); Centers for Disease Control and Prevention, Fort Collins, Colorado, USA (A. Lambert, R. Lanciotti); Patología Hospital Universitario de Neiva, Neiva, Colombia (F. Bolaños); Instituto Nacional de Salud, Bogota, Colombia (E.A. Parra Saad)

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Table 3

Characteristics and laboratory findings for 13 microcephaly-associated case-patients*

Case-patient no. Maternal travel history or residence Maternal symptom onset, gestation wk/trimester Outcome End of pregnancy, gestational age, wk/trimester Results of Zika virus testing performed on FFPE tissues
RT-PCR ISH†
54 Brazil 4/first Infant with microcephaly died 6 h after birth 38/third Positive (brain); negative (placenta, spleen, kidney, lung, liver) Positive (brain)
66 Colombia 8/first Infant with microcephaly died 2 d after birth 26/third Positive (brain, placenta); negative (liver) Positive (brain)
67 Colombia 8/first Infant with microcephaly died shortly after birth 27/third Positive (brain, placenta); negative (liver) Positive (brain)
68 Colombia 10/first Infant with microcephaly died shortly after birth 27/third Positive (brain, placenta); negative (liver) Positive (brain), negative (placenta)
55 Brazil NA/first Infant with microcephaly died few min after birth 29/third Positive (brain); negative (placenta) Positive (brain)
53 Brazil NA/first Infant with microcephaly died 20 h after birth 36/third Positive (brain); negative (placenta, spleen, kidney, heart) Positive (brain)
37 Brazil NA/first Infant with microcephaly died 60 d after birth 38/third Positive (brain) Positive (brain)
65 Colombia NA/first Infant with microcephaly died few minutes after birth 28/third Positive (brain, placenta); negative (liver) Negative (brain, placenta)
49 Brazil, delivered in USA 7/first Infant with nonfatal microcephaly 37/third Positive (placenta) Tissue NA
83 Cape Verde‡ 7/first Infant with nonfatal microcephaly 36/third Positive (placenta) Negative (placenta)
20 Marshall Islands‡ Unknown Infant with nonfatal microcephaly 31/third Positive (placenta) Tissue NA
85 Honduras‡ 10/first Infant with nonfatal microcephaly 37/third Positive (placenta, umbilical cord) Negative (placenta)
13 Dominican Republic 18/second Infant with nonfatal microcephaly 39/third Negative (placenta, umbilical cord, membrane) ND

*FFPE, formalin-fixed, paraffin-embedded; ISH, in situ hybridization; NA, not available; ND, not done; RT-PCR, reverse transcription PCR.
†ISH results include staining by sense and antisense probes.
‡Travel history.

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