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Volume 23, Number 3—March 2017

Research

Zika Virus RNA Replication and Persistence in Brain and Placental Tissue

Julu BhatnagarComments to Author , Demi B. Rabeneck, Roosecelis B. Martines, Sarah Reagan-Steiner, Yokabed Ermias, Lindsey B.C. Estetter, Tadaki Suzuki, Jana M. Ritter, M. Kelly Keating, Gillian Hale, Joy Gary, Atis Muehlenbachs, Amy J. Lambert, Robert Lanciotti, Titilope Oduyebo, Dana Meaney-Delman, Fernando Bolaños, Edgar Alberto Parra Saad, Wun-Ju Shieh, and Sherif Zaki
Author affiliations: Centers for Disease Control and Prevention, Atlanta, Georgia, USA (J. Bhatnagar, D.B. Rabeneck, R.B. Martines, S. Reagan-Steiner, Y. Ermias, L.B.C. Estetter, T. Suzuki, J. Ritter, M.K. Keating, G. Hale, J. Gary, A. Muehlenbachs, T. Oduyebo, D. Meaney-Delman, W. Shieh, S.R. Zaki); Centers for Disease Control and Prevention, Fort Collins, Colorado, USA (A. Lambert, R. Lanciotti); Patología Hospital Universitario de Neiva, Neiva, Colombia (F. Bolaños); Instituto Nacional de Salud, Bogota, Colombia (E.A. Parra Saad)

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Table 3

Characteristics and laboratory findings for 13 microcephaly-associated case-patients*

Case-patient no. Maternal travel history or residence Maternal symptom onset, gestation wk/trimester Outcome End of pregnancy, gestational age, wk/trimester Results of Zika virus testing performed on FFPE tissues
RT-PCR ISH†
54 Brazil 4/first Infant with microcephaly died 6 h after birth 38/third Positive (brain); negative (placenta, spleen, kidney, lung, liver) Positive (brain)
66 Colombia 8/first Infant with microcephaly died 2 d after birth 26/third Positive (brain, placenta); negative (liver) Positive (brain)
67 Colombia 8/first Infant with microcephaly died shortly after birth 27/third Positive (brain, placenta); negative (liver) Positive (brain)
68 Colombia 10/first Infant with microcephaly died shortly after birth 27/third Positive (brain, placenta); negative (liver) Positive (brain), negative (placenta)
55 Brazil NA/first Infant with microcephaly died few min after birth 29/third Positive (brain); negative (placenta) Positive (brain)
53 Brazil NA/first Infant with microcephaly died 20 h after birth 36/third Positive (brain); negative (placenta, spleen, kidney, heart) Positive (brain)
37 Brazil NA/first Infant with microcephaly died 60 d after birth 38/third Positive (brain) Positive (brain)
65 Colombia NA/first Infant with microcephaly died few minutes after birth 28/third Positive (brain, placenta); negative (liver) Negative (brain, placenta)
49 Brazil, delivered in USA 7/first Infant with nonfatal microcephaly 37/third Positive (placenta) Tissue NA
83 Cape Verde‡ 7/first Infant with nonfatal microcephaly 36/third Positive (placenta) Negative (placenta)
20 Marshall Islands‡ Unknown Infant with nonfatal microcephaly 31/third Positive (placenta) Tissue NA
85 Honduras‡ 10/first Infant with nonfatal microcephaly 37/third Positive (placenta, umbilical cord) Negative (placenta)
13 Dominican Republic 18/second Infant with nonfatal microcephaly 39/third Negative (placenta, umbilical cord, membrane) ND

*FFPE, formalin-fixed, paraffin-embedded; ISH, in situ hybridization; NA, not available; ND, not done; RT-PCR, reverse transcription PCR.
†ISH results include staining by sense and antisense probes.
‡Travel history.

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Page created: February 17, 2017
Page updated: February 17, 2017
Page reviewed: February 17, 2017
The conclusions, findings, and opinions expressed by authors contributing to this journal do not necessarily reflect the official position of the U.S. Department of Health and Human Services, the Public Health Service, the Centers for Disease Control and Prevention, or the authors' affiliated institutions. Use of trade names is for identification only and does not imply endorsement by any of the groups named above.
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