Volume 23, Number 3—March 2017
Research
Zika Virus RNA Replication and Persistence in Brain and Placental Tissue
Table 3
Characteristics and laboratory findings for 13 microcephaly-associated case-patients*
Case-patient no. | Maternal travel history or residence | Maternal symptom onset, gestation wk/trimester | Outcome | End of pregnancy, gestational age, wk/trimester | Results of Zika virus testing performed on FFPE tissues |
|
---|---|---|---|---|---|---|
RT-PCR | ISH† | |||||
54 | Brazil | 4/first | Infant with microcephaly died 6 h after birth | 38/third | Positive (brain); negative (placenta, spleen, kidney, lung, liver) | Positive (brain) |
66 | Colombia | 8/first | Infant with microcephaly died 2 d after birth | 26/third | Positive (brain, placenta); negative (liver) | Positive (brain) |
67 | Colombia | 8/first | Infant with microcephaly died shortly after birth | 27/third | Positive (brain, placenta); negative (liver) | Positive (brain) |
68 | Colombia | 10/first | Infant with microcephaly died shortly after birth | 27/third | Positive (brain, placenta); negative (liver) | Positive (brain), negative (placenta) |
55 | Brazil | NA/first | Infant with microcephaly died few min after birth | 29/third | Positive (brain); negative (placenta) | Positive (brain) |
53 | Brazil | NA/first | Infant with microcephaly died 20 h after birth | 36/third | Positive (brain); negative (placenta, spleen, kidney, heart) | Positive (brain) |
37 | Brazil | NA/first | Infant with microcephaly died 60 d after birth | 38/third | Positive (brain) | Positive (brain) |
65 | Colombia | NA/first | Infant with microcephaly died few minutes after birth | 28/third | Positive (brain, placenta); negative (liver) | Negative (brain, placenta) |
49 | Brazil, delivered in USA | 7/first | Infant with nonfatal microcephaly | 37/third | Positive (placenta) | Tissue NA |
83 | Cape Verde‡ | 7/first | Infant with nonfatal microcephaly | 36/third | Positive (placenta) | Negative (placenta) |
20 | Marshall Islands‡ | Unknown | Infant with nonfatal microcephaly | 31/third | Positive (placenta) | Tissue NA |
85 | Honduras‡ | 10/first | Infant with nonfatal microcephaly | 37/third | Positive (placenta, umbilical cord) | Negative (placenta) |
13 | Dominican Republic | 18/second | Infant with nonfatal microcephaly | 39/third | Negative (placenta, umbilical cord, membrane) | ND |
*FFPE, formalin-fixed, paraffin-embedded; ISH, in situ hybridization; NA, not available; ND, not done; RT-PCR, reverse transcription PCR.
†ISH results include staining by sense and antisense probes.
‡Travel history.