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Volume 23, Number 9—September 2017

Research

Protective Effect of Val129-PrP against Bovine Spongiform Encephalopathy but not Variant Creutzfeldt-Jakob Disease

Natalia Fernández-Borges1, Juan Carlos Espinosa1, Alba Marín-Moreno, Patricia Aguilar-Calvo, Emmanuel A. Asante, Tetsuyuki Kitamoto, Shirou Mohri, Olivier Andréoletti, and Juan María TorresComments to Author 
Author affiliations: Centro de Investigación en Sanidad Animal, Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (CISA-INIA); Valdeolmos, Madrid, Spain (N. Fernández-Borges, J.C. Espinosa, A. Marín-Moreno, P. Aguilar-Calvo, J.M. Torres); MRC Prion Unit, Department of Neurodegenerative Disease, University College London, Institute of Neurology, London, UK (E.A. Asante); Tohoku University Graduate School of Medicine, Sendai, Japan (T. Kitamoto, S. Mohri); UMR INRA ENVT 1225, Interactions Hôtes Agents Pathogènes, Ecole Nationale Vétérinaire de Toulouse, Toulouse, France (O. Andréoletti)

Main Article

Figure 1

Biochemical features of the protease-resistant prion protein (PrPres) detected in the brain of TgMet129, TgMet/Val129, and TgVal129 mice inoculated with vCJD. A) PrPres detected in TgMet129 (lanes 2 and 5), TgMet/Val129 (lane 3), and TgVal129 (lane 4 and 6) mice inoculated with vCJD brain homogenate or TgMet129-passaged vCJD prions. Similar quantities of PrPres were loaded for adequate comparison and immunoblots were detected with Sha31 monoclonal antibody (mAb). The original vCJD isolate (Hu-vC

Figure 1. Biochemical features of the protease-resistant prion protein (PrPres) detected in the brain of TgMet129, TgMet/Val129, and TgVal129 mice inoculated with vCJD. A) PrPres detected in TgMet129 (lanes 2 and 5), TgMet/Val129 (lane 3), and TgVal129 (lanes 4 and 6) mice inoculated with vCJD brain homogenate or TgMet129-passaged vCJD prions. Similar quantities of PrPres were loaded for adequate comparison and immunoblots were detected with Sha31 monoclonal antibody (mAb). The original vCJD isolate (Hu-vCJD2) used for mouse inoculations was also included in the blot (lanes 1 and 7). sCJD VV2 and MM1 isolates were included for biochemical comparative purposes (lanes 8 and 9, respectively). Molecular weight (MW) in kDa is shown. B) Glycoform analysis of PrPres from TgMet129, TgMet/Val129 and TgVal129 mice inoculated with vCJD brain homogenate or TgMet129-passaged vCJD prions. PrPres was detected by Western blot testing using the Sha31 mAb, as for panel A. The data shown are the means of >4 measurements in >2 different Western blots using the Image Lab (Bio-Rad, Hercules, CA, USA) program after capture with ChemiDoc XRS+ (Bio-Rad) under nonsaturating conditions. Error bars indicate SD. CJD, Creutzfeldt-Jakob disease; sCJD, sporadic CJD; vCJD, variant CJD.

Main Article

1These authors contributed equally to this article.

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