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Volume 23, Number 9—September 2017

Research

Protective Effect of Val129-PrP against Bovine Spongiform Encephalopathy but not Variant Creutzfeldt-Jakob Disease

Natalia Fernández-Borges1, Juan Carlos Espinosa1, Alba Marín-Moreno, Patricia Aguilar-Calvo, Emmanuel A. Asante, Tetsuyuki Kitamoto, Shirou Mohri, Olivier Andréoletti, and Juan María TorresComments to Author 
Author affiliations: Centro de Investigación en Sanidad Animal, Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (CISA-INIA); Valdeolmos, Madrid, Spain (N. Fernández-Borges, J.C. Espinosa, A. Marín-Moreno, P. Aguilar-Calvo, J.M. Torres); MRC Prion Unit, Department of Neurodegenerative Disease, University College London, Institute of Neurology, London, UK (E.A. Asante); Tohoku University Graduate School of Medicine, Sendai, Japan (T. Kitamoto, S. Mohri); UMR INRA ENVT 1225, Interactions Hôtes Agents Pathogènes, Ecole Nationale Vétérinaire de Toulouse, Toulouse, France (O. Andréoletti)

Main Article

Table 3

Intracerebral inoculation of transgenic mice that express human PrP with vCJD and with vCJD previously adapted in TgMet129 or TgMet/Val129 mice*

Isolates
Mean survival time, d ±SD (no. PrPres-positive/inoculated animals) [reference]†
TgMet129
TgMet/Val129
TgVal129
Hu-vCJD1 626 ±29 (6/6) [31] >700‡ (3/3)§ >700‡ (0/5)
Hu-vCJD1→TgMet129 650 ±60 (4/4) >700‡ (5/5) >700‡ (5/5)
Hu-vCJD2 545 ±146 (5/5) >700‡ (5/5) >700‡ (0/6)#
Hu-vCJD2→TgMet129 564 ±39 (4/4) >700‡ (5/5) >700‡ (2/2)¶
Hu-vCJD2→TgMet/Val129 601 ±32 (5/5) 651 ±17 (7/7) >700‡ (7/7)
Ca-BSE2→TgMet129 614 ±87 (6/6) >700‡ (4/4) >700‡ (3/4)
Ca-BSE/Sh(ARQ)→TgMet129 534 ±55 (5/6) >700‡ (5/6) >700‡ (5/6)
Ca-BSE/Go→TgMet129 609 ±67 (5/5) >700‡ (4/4) >700‡ (6/6)

*BSE, bovine spongiform encephalopathy; Ca, cattle; CJD, Creutzfeldt-Jakob disease; Go, goat; Hu, human; Met129, methionine; PrP, prion protein; PrPres, proteinase K–resistant PrP; sCJD, sporadic CJD; Val129, valine; vCJD, variant CJD.
†Survival time is indicated for all mice scored positive for PrPres.
‡Animals were euthanized without clinical signs at 700 dpi.
§Three additional animals had to be culled before the end of the experiment because of intercurrent illnesses; all were negative for brain PrPres on WB.
¶Four additional animals had to be culled before the end of the experiment because of intercurrent illnesses; all were negative for PrPres expression on Western blot.
#Positive subclinical infection tested in Bo-Tg110 mice.

Main Article

1These authors contributed equally to this article.

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