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Volume 23, Number 9—September 2017
Research

Protective Effect of Val129-PrP against Bovine Spongiform Encephalopathy but not Variant Creutzfeldt-Jakob Disease

Natalia Fernández-Borges1, Juan Carlos Espinosa1, Alba Marín-Moreno, Patricia Aguilar-Calvo, Emmanuel A. Asante, Tetsuyuki Kitamoto, Shirou Mohri, Olivier Andréoletti, and Juan María TorresComments to Author 
Author affiliations: Centro de Investigación en Sanidad Animal, Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (CISA-INIA); Valdeolmos, Madrid, Spain (N. Fernández-Borges, J.C. Espinosa, A. Marín-Moreno, P. Aguilar-Calvo, J.M. Torres); MRC Prion Unit, Department of Neurodegenerative Disease, University College London, Institute of Neurology, London, UK (E.A. Asante); Tohoku University Graduate School of Medicine, Sendai, Japan (T. Kitamoto, S. Mohri); UMR INRA ENVT 1225, Interactions Hôtes Agents Pathogènes, Ecole Nationale Vétérinaire de Toulouse, Toulouse, France (O. Andréoletti)

Main Article

Table 2

Transmission of cattle, porcine, sheep, and goat BSE isolates to mice in transgenic mouse lines TgMet129, TgMet/Val129, and TgVal129*

Isolates
 Mean survival time, d ±SD (no. PrPres-positive/inoculated animals) [reference]†‡
TgMet129
 TgMet/Val129
 TgVal129
First passage
 Second passage
 First passage
 Second passage
 First passage
 Second passage
Hu-sCJD MM1 219 ±17 (6/6) [40] 239 ±8 (6/6) [40] 243 ±14 (6/6) [40] 260 ±13 (6/6) [40] 327 ±19 (6/6) [40] 286 ±16 (6/6) [40]
Hu-sCJD VV2 618 ±81 (6/6) [40] 509 ±41 (6/6) [40] 588 ±74 (6/6) [40] 594 ±86 (6/6) [40] 168 ±12 (6/6) [40] 169 ±12 (6/6) [40]
Hu-TSE negative >700‡ (0/6) >700‡ (0/6) >700‡ (0/6) >700‡ (0/6) >700‡ (0/6) >700‡ (0/6)
Ca-BSE0 739 (1/6) (31) 633 ±32 (6/6) >700‡ (0/6) >700‡ (0/6) >700‡ (0/6) >700‡ (0/6)
Ca-BSE2 491–707 (0/9) [31] 572 ±37 (3/4) [31] >700‡ (0/5) ND >700‡ (0/3)§ >700‡ (0/3)§
Ca-BSE/TgPo 653 ±45 (3/5) ND ND ND >700‡ (0/6) ND
Ca-BSE/Sh(ARQ) 615 ±84 (4/6) [31] 564 ±39 (5/5) [31] >700b (0/6) >700‡ (0/6) >700‡ (0/6) >700‡ (0/6)
Ca-BSE/Go >700‡ (5/5) >700‡ (5/5) 476 (1/10) ND >700‡ (0/5)¶ ND
Go-BSE 683 ± 36 (6/6) 675 ± 36 (5/5) >700‡ (0/6) ND >700‡ (0/6) ND

*BSE, bovine spongiform encephalopathy; Ca, cattle; CJD, Creutzfeldt-Jakob disease; Go, goat; Hu, human; Met129, methionine; ND, not done; PrPres, proteinase K–resistant PrP; sCJD, sporadic CJD; Val129, valine; vCJD, variant CJD.
†Survival time is indicated for all mice that scored positive for PrPres
‡Animals without clinical signs were euthanized at 700 dpi.
§Three additional animals were culled before the end of the experiment because of intercurrent illnesses; all were negative for brain PrPres by using Western blot.
¶Positive subclinical infection tested in Bo-Tg110 mice.

Main Article

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1These authors contributed equally to this article.

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