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Volume 24, Number 8—August 2018

Human Norovirus Replication in Human Intestinal Enteroids as Model to Evaluate Virus Inactivation

Veronica CostantiniComments to Author , Esther K. Morantz, Hannah Browne, Khalil Ettayebi, Xi-Lei Zeng, Robert L. Atmar, Mary K. Estes, and Jan Vinjé
Author affiliations: Centers for Disease Control and Prevention, Atlanta, Georgia, USA (V. Costantini, J. Vinjé); Synergy America, Inc., Atlanta (E.K. Morantz); Oak Ridge Institute for Science and Education, Oak Ridge, Tennessee, USA (H. Browne); Baylor College of Medicine, Houston, Texas, USA (K. Ettayebi, X.-L. Zeng, R.L. Atmar, M.K. Estes)

Main Article

Table 2

Norovirus fecal filtrates successfully cultivated in study of human norovirus replication in human intestinal enteroids

Sample ID Genotype, RdRp-Capsid* Mean norovirus RNA copies/μL Participant age, mo Collection date† Storage condition‡ Fold virus RNA increase, log10§
NP01015 GII.Pg-GII.1 1.4 × 105 4 2017 Mar −70°C 696 (2.8)
NP36980 GII.P16-GII.2 1.9 × 103 132 2017 Feb −70°C 56 (1.8)
N741656 GII.P12-GII.3 1.4 × 105 9 2012 Aug −70°C 181 (2.3)
G3868 GII.P4 Den Haag-GII.4 Den Haag 2.0 × 105 12 2015 May −70°C 155 (2.2)
G3829 GII.P4 New Orleans-GII.4 New Orleans 4.1 × 105 5 2013 Apr −70°C 227 (2.4)
A5413 GII.Pe-GII.4 Sydney 1.6 × 106 12 2012 −70°C 487 (2.7)
R3702 GII.Pe-GII.4 Sydney 7.7 × 106 46 2015 Jan −70°C 1236 (3.0)
V0882 GII.Pe-GII.4 Sydney 4.0 × 106 20 2015 Feb −70°C 998 (3.0)
V0900 GII.Pe-GII.4 Sydney 5.1 × 105 17 2015 Feb −70°C 1102 (3.0)
H4423 GII.Pe-GII.4 Sydney 5.9 × 105 12 2015 Feb −70°C 125 (2.1)
H4587 GII.Pe-GII.4 Sydney 4.4 × 106 21 2015 Apr −70°C 448 (2.7)
C8993 GII.Pe-GII.4 Sydney 1.7 × 107 19 2015 Mar −70°C 223 (2.3)
CDC830¶ GII.Pe-GII.4 Sydney 2.9 × 106 996 2016 Apr 4°C 121 (2.1)
NP36995 GII.P16-GII.4 Sydney 2.1 × 106 14 2016 Dec −70°C 340 (2.5)
NP36994 GII.P7-GII.14 6.1 × 104 22 2016 Dec −70°C 47 (1.6)
G3734 GII.Pe-GII.17 3.4 × 105 11 2010 Oct −70°C 96 (1.9)

*Dual genotyping based on sequencing partial RdRp and capsid regions (26,27). RdRp, RNA-dependent RNA polymerase.
†When month was not available, only year of collection is reported.
‡Samples were kept from collection time until testing at the indicated temperature.
§Virus RNA increase after 3 days postinfection expressed as fold increase and log10 fold increase. For each fecal filtrate, data represent mean viral RNA increase from 3 experiments with 3 technical replicates each.
¶CDC830 was stored at 4°C for 2 mo before cultivation.

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