Figure 3. Representative paraffin-embedded tissue (PET) blots of protease-resistant, disease-related prion protein (resPrPD) distribution in phenotypes T1–T3 and controls. A) For T1, PrPD predominated in cerebral cortex (C), thalamus (T), superior colliculus (SC), lateral geniculate nucleus (LG), and substantia nigra (SN). A similar PrPD distribution was observed with transmission of sCJDMV1 used as type control. T2 showed a more uniform distribution in cerebral cortex and subcortical nuclei of an apparently lesser amount of PrPD; T3 appeared to preferentially affect the hemispheric white matter and other subcortical regions such as the alveus, the corpus callosum, the anterior commissure, and fascicles surrounding thalamus as well as other white matter formations such as fimbria, brachium of superior colliculus, medial lemniscus, and cerebral peduncles. Small amounts of PrPD were also observed in cerebellar and medullary white matter (asterisk [*]). B) PrPD T1 distribution resembled that of bank voles (bv) 109I after transmission of the same VPSPr-MV brain homogenate (compare with T1 in A). A similar distribution was also observed after inoculation with sCJDMV1. Left to right: coronal sections of telencephalon midlevel caudate nucleus; diencephalon midlevel thalamus; midbrain; and hindbrain-level medulla and cerebellum. sCJD, sporadic Creutzfeldt-Jakob disease; VPSPr, variably protease sensitive prionopathy.