Variable Protease-Sensitive Prionopathy Transmission to Bank Voles
Romolo Nonno
1, Silvio Notari
1, Michele Angelo Di Bari, Ignazio Cali, Laura Pirisinu, Claudia d’Agostino, Laura Cracco, Diane Kofskey, Ilaria Vanni, Jody Lavrich, Piero Parchi, Umberto Agrimi, and Pierluigi Gambetti
Author affiliations: Istituto Superiore di Sanità, Rome, Italy (R. Nonno, M.A. Di Bari, L. Pirisinu, C. d’Agostino, I. Vanni, U. Agrimi); Case Western Reserve University, Cleveland, Ohio, USA (S. Notari, I. Cali, L. Cracco, D. Kofskey, J. Lavrich, P. Gambetti); University of Bologna, Bologna, Italy (P. Parchi); Istituto delle Scienze Neurologiche di Bologna, Bologna (P. Parchi)
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Figure 6
Figure 6. Relative quantities of totPrPD and resPrPD in T1–T3 phenotypes. totPrPD accounted for 93.1% and resPrPD for 81.3% of total PrP recovered from bank voles harboring the T1 phenotype. Corresponding percentages for T2 were 91.0% and 33.0%, and T3 totPrPD and resPrPD accounted only for 8.0% and 0.2% of total PrP and differed significantly from both T1 and T2 in each of the 2 components. ResPrPD also differed significantly between T1 and T2 (each bar represents the mean ± SD of n = 3 T1, n = 3 T2, and n = 5 T3; all data are from bank voles 109I; antibody 9A2). bv, bank vole; resPrPD protease-resistant, disease-related prion protein; totPrPD, comprising protease-sensitive PrPD and resPrPD. *p<0.0001; †p<0.0001 vs. T1 and p<0.05 vs. T2; ‡p<0.01.
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