Figure 5. Glycoform ratio of protease-resistant, disease-related prion protein (resPrPD) in phenotypes T1 and T2. The ratio of resPrPD associated with T1 (T1 109I) was 48% for diglycosylated, 44% for monoglycosylated, and 8% for unglycosylated conformers and significantly differed in each glycoform from the 17%, 63%, and 20% corresponding ratio of T2 (T2 109I). *p<0.0001; †p<0.005; ‡p<0.05). Glycoform ratios of T1 109I and T1 109M as well as that of type 1 control (from bank voles 109I inoculated with sporadic Creutzfeldt-Jakob disease MV1 and used as human type 1 controls) did not significantly differ from each other. Each bar represents mean ± SD of n = 4 for T1 109M, n = 6 for T1 109I, n = 6 for T2, and n = 2 for type 1 control.