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Volume 26, Number 1—January 2020
Synopsis

Candidatus Mycoplasma haemohominis in Human, Japan

Norimichi Hattori1Comments to Author , Makoto Kuroda1, Harutaka Katano1, Takahiro Takuma1, Takayoshi Ito1, Nana Arai, Ryo Yanai, Tsuyoshi Sekizuka, Sho Ishii, Yoko Miura, Takahiro Tokunaga, Hiroyuki Watanabe, Norihiro Nomura, Junichi Eguchi, Hideki Hasegawa, Tsuyoshi Nakamaki, Takaji Wakita, and Yoshihito Niki
Author affiliations: Showa University School of Medicine, Tokyo, Japan (N. Hattori, T. Takuma, N. Arai, R. Yanai, S. Ishii, Y. Miura, T. Tokunaga, T. Nakamaki, Y. Niki); National Institute of Infectious Diseases, Tokyo (M. Kuroda, H. Katano, T. Sekizuka, H. Hasegawa, T. Wakita); Showa Uuniversity Koto Toyosu Hospital, Tokyo (T. Ito, H. Watanabe, N. Nomura, J. Eguchi)

Main Article

Figure 8

Detection of a gyrA mutation in the QFDR of Candidatus Mycoplasma haemohominis genome isolated from in a 42-year-old man, Japan, who was given levofloxacin. Read-mapping analysis identified a nonsynonymous amino acid substitution in gyrA for quinolone-resistant Candidatus M. haemohominis from a serum sample. Bottom panel shows a schematic of the gyrA amino acid sequence and alignment with those of other QRDRs (17,18). Dots indicate identical amino acids corresponding to the Candidatus M. haemoho

Figure 8. Detection of a gyrA mutation in the QFDR of Candidatus Mycoplasma haemohominis genome isolated from in a 42-year-old man, Japan, who was given levofloxacin. Read-mapping analysis identified a nonsynonymous amino acid substitution in gyrA for quinolone-resistant Candidatus M. haemohominis from a serum sample. Bottom panel shows a schematic of the gyrA amino acid sequence and alignment with those of other QRDRs (17,18). Dots indicate identical amino acids corresponding to the Candidatus M. haemohominis sequence. Red indicates nucleotide or amino acid mutations. Arrow indicates direction of transcription. QRDR, quinolone resistance–determining region.

Main Article

References
  1. Messick  JB. Hemotrophic mycoplasmas (hemoplasmas): a review and new insights into pathogenic potential. Vet Clin Pathol. 2004;33:2–13. DOIPubMedGoogle Scholar
  2. Harasawa  R, Fujita  H, Kadosaka  T, Ando  S, Rikihisa  Y. Proposal for ‘Candidatus Mycoplasma haemomuris subsp. musculi’ in mice, and ‘Candidatus Mycoplasma haemomuris subsp. ratti’ in rats. Int J Syst Evol Microbiol. 2015;65:734–7. DOIPubMedGoogle Scholar
  3. Iso  T, Suzuki  J, Sasaoka  F, Sashida  H, Watanabe  Y, Fujihara  M, et al. Hemotropic mycoplasma infection in wild black bears (Ursus thibetanus japonicus). Vet Microbiol. 2013;163:184–9. DOIPubMedGoogle Scholar
  4. Millán  J, López-Roig  M, Delicado  V, Serra-Cobo  J, Esperón  F. Widespread infection with hemotropic mycoplasmas in bats in Spain, including a hemoplasma closely related to “Candidatus Mycoplasma hemohominis”. Comp Immunol Microbiol Infect Dis. 2015;39:9–12. DOIPubMedGoogle Scholar
  5. Sykes  JE. Feline hemotropic mycoplasmas. Vet Clin North Am Small Anim Pract. 2010;40:1157–70. DOIPubMedGoogle Scholar
  6. dos Santos  AP, dos Santos  RP, Biondo  AW, Dora  JM, Goldani  LZ, de Oliveira  ST, et al. Hemoplasma infection in HIV-positive patient, Brazil. Emerg Infect Dis. 2008;14:1922–4. DOIPubMedGoogle Scholar
  7. Yuan  CL, Liang  AB, Yao  CB, Yang  ZB, Zhu  JG, Cui  L, et al. Prevalence of Mycoplasma suis (Eperythrozoon suis) infection in swine and swine-farm workers in Shanghai, China. Am J Vet Res. 2009;70:890–4. DOIPubMedGoogle Scholar
  8. Sykes  JE, Lindsay  LL, Maggi  RG, Breitschwerdt  EB. Human coinfection with Bartonella henselae and two hemotropic mycoplasma variants resembling Mycoplasma ovis. J Clin Microbiol. 2010;48:3782–5. DOIPubMedGoogle Scholar
  9. Steer  JA, Tasker  S, Barker  EN, Jensen  J, Mitchell  J, Stocki  T, et al. A novel hemotropic Mycoplasma (hemoplasma) in a patient with hemolytic anemia and pyrexia. Clin Infect Dis. 2011;53:e147–51. DOIPubMedGoogle Scholar
  10. Maggi  RG, Mascarelli  PE, Havenga  LN, Naidoo  V, Breitschwerdt  EB. Co-infection with Anaplasma platys, Bartonella henselae and Candidatus Mycoplasma haematoparvum in a veterinarian. Parasit Vectors. 2013;6:103. DOIPubMedGoogle Scholar
  11. Maggi  RG, Compton  SM, Trull  CL, Mascarelli  PE, Mozayeni  BR, Breitschwerdt  EB. Infection with hemotropic Mycoplasma species in patients with or without extensive arthropod or animal contact. J Clin Microbiol. 2013;51:3237–41. DOIPubMedGoogle Scholar
  12. Yang  D, Tai  X, Qiu  Y, Yun  S. Prevalence of Eperythrozoon spp. infection and congenital eperythrozoonosis in humans in Inner Mongolia, China. Epidemiol Infect. 2000;125:421–6. DOIPubMedGoogle Scholar
  13. Takeuchi  F, Sekizuka  T, Yamashita  A, Ogasawara  Y, Mizuta  K, Kuroda  M. MePIC, metagenomic pathogen identification for clinical specimens. Jpn J Infect Dis. 2014;67:62–5. DOIPubMedGoogle Scholar
  14. Huson  DH, Beier  S, Flade  I, Górska  A, El-Hadidi  M, Mitra  S, et al. MEGAN community edition: interactive exploration and analysis of large-scale microbiome sequencing data. PLOS Comput Biol. 2016;12:e1004957. DOIPubMedGoogle Scholar
  15. Li  H, Durbin  R. Fast and accurate long-read alignment with Burrows-Wheeler transform. Bioinformatics. 2010;26:589–95. DOIPubMedGoogle Scholar
  16. Tasker  S, Peters  IR, Mumford  AD, Day  MJ, Gruffydd-Jones  TJ, Day  S, et al. Investigation of human haemotropic Mycoplasma infections using a novel generic haemoplasma qPCR assay on blood samples and blood smears. J Med Microbiol. 2010;59:1285–92. DOIPubMedGoogle Scholar
  17. Murray  GL, Bradshaw  CS, Bissessor  M, Danielewski  J, Garland  SM, Jensen  JS, et al. Increased macrolide and fluoroquinolone resistance in Mycoplasma genitalium. Emerg Infect Dis. 2017;23:809–12. DOIPubMedGoogle Scholar
  18. Fricke  WF, Wright  MS, Lindell  AH, Harkins  DM, Baker-Austin  C, Ravel  J, et al. Insights into the environmental resistance gene pool from the genome sequence of the multidrug-resistant environmental isolate Escherichia coli SMS-3-5. J Bacteriol. 2008;190:6779–94. DOIPubMedGoogle Scholar
  19. Novacco  M, Sugiarto  S, Willi  B, Baumann  J, Spiri  AM, Oestmann  A, et al. Consecutive antibiotic treatment with doxycycline and marbofloxacin clears bacteremia in Mycoplasma haemofelis-infected cats. Vet Microbiol. 2018;217:112–20. DOIPubMedGoogle Scholar
  20. Hamasuna  R, Le  PT, Kutsuna  S, Furubayashi  K, Matsumoto  M, Ohmagari  N, et al. Mutations in ParC and GyrA of moxifloxacin-resistant and susceptible Mycoplasma genitalium strains. PLoS One. 2018;13:e0198355. DOIPubMedGoogle Scholar
  21. Weigel  LM, Steward  CD, Tenover  FC. gyrA mutations associated with fluoroquinolone resistance in eight species of Enterobacteriaceae. Antimicrob Agents Chemother. 1998;42:2661–7. DOIPubMedGoogle Scholar
  22. Tasker  S, Peters  IR, Papasouliotis  K, Cue  SM, Willi  B, Hofmann-Lehmann  R, et al. Description of outcomes of experimental infection with feline haemoplasmas: copy numbers, haematology, Coombs’ testing and blood glucose concentrations. Vet Microbiol. 2009;139:323–32. DOIPubMedGoogle Scholar
  23. Barker  EN, Darby  AC, Helps  CR, Peters  IR, Heesom  KJ, Arthur  CJ, et al. Molecular characterization of the uncultivatable hemotropic bacterium Mycoplasma haemofelis. Vet Res (Faisalabad). 2011;42:83. DOIPubMedGoogle Scholar
  24. Mohri  H. Acquired von Willebrand syndrome: features and management. Am J Hematol. 2006;81:616–23. DOIPubMedGoogle Scholar
  25. Tiede  A, Rand  JH, Budde  U, Ganser  A, Federici  AB. How I treat the acquired von Willebrand syndrome. Blood. 2011;117:6777–85. DOIPubMedGoogle Scholar

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1These authors contributed equally to this article.

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Page updated: December 18, 2019
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