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Volume 26, Number 8—August 2020
Synopsis

US CDC Real-Time Reverse Transcription PCR Panel for Detection of Severe Acute Respiratory Syndrome Coronavirus 2

Xiaoyan Lu, Lijuan Wang, Senthilkumar K. Sakthivel, Brett Whitaker, Janna Murray, Shifaq Kamili, Brian Lynch, Lakshmi Malapati, Stephen A. Burke, Jennifer Harcourt, Azaibi Tamin, Natalie J. Thornburg, Julie M. Villanueva, and Stephen LindstromComments to Author 
Author affiliations: Centers for Disease Control and Prevention, Atlanta, Georgia, USA (X. Lu, B. Whitaker, S.A. Burke, J. Harcourt, A. Tamin, N.J. Thornburg, J.M. Villanueva, S. Lindstrom); Synergy America, Inc., Atlanta (L. Wang, S. Kamili); Eagle Contracting, Atlanta (S.K. Sakthivel, J. Murray, B. Lynch); Leidos, Atlanta (L. Malapati); Battelle, Atlanta (S.A. Burke)

Main Article

Figure 1

Linear regression analysis of serial 10-fold dilutions of synthetic RNA transcripts of the nucleocapsid gene (N) ranging from 5 to 5 × 107 copies/reaction tested by the N1 (A), N2 (B), and N3 (C) assays in the US Centers for Disease Control and Prevention real-time reverse transcription PCR panel for detection of severe acute respiratory syndrome coronavirus 2. For each assay, R2 indicates calculated linear correlation coefficients and eff. indicates amplification efficiencies. Ct, cycle thresho

Figure 1. Linear regression analysis of serial 10-fold dilutions of synthetic RNA transcripts of the nucleocapsid gene (N) ranging from 5 to 5 × 107 copies/reaction tested by the N1 (A), N2 (B), and N3 (C) assays in the US Centers for Disease Control and Prevention real-time reverse transcription PCR panel for detection of severe acute respiratory syndrome coronavirus 2. For each assay, R2 indicates calculated linear correlation coefficients and eff. indicates amplification efficiencies. Ct, cycle threshold.

Main Article

Page created: May 14, 2020
Page updated: July 17, 2020
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The conclusions, findings, and opinions expressed by authors contributing to this journal do not necessarily reflect the official position of the U.S. Department of Health and Human Services, the Public Health Service, the Centers for Disease Control and Prevention, or the authors' affiliated institutions. Use of trade names is for identification only and does not imply endorsement by any of the groups named above.
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