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Volume 26, Number 8—August 2020
Synopsis

US CDC Real-Time Reverse Transcription PCR Panel for Detection of Severe Acute Respiratory Syndrome Coronavirus 2

Xiaoyan Lu, Lijuan Wang, Senthilkumar K. Sakthivel, Brett Whitaker, Janna Murray, Shifaq Kamili, Brian Lynch, Lakshmi Malapati, Stephen A. Burke, Jennifer Harcourt, Azaibi Tamin, Natalie J. Thornburg, Julie M. Villanueva, and Stephen LindstromComments to Author 
Author affiliations: Centers for Disease Control and Prevention, Atlanta, Georgia, USA (X. Lu, B. Whitaker, S.A. Burke, J. Harcourt, A. Tamin, N.J. Thornburg, J.M. Villanueva, S. Lindstrom); Synergy America, Inc., Atlanta (L. Wang, S. Kamili); Eagle Contracting, Atlanta (S.K. Sakthivel, J. Murray, B. Lynch); Leidos, Atlanta (L. Malapati); Battelle, Atlanta (S.A. Burke)

Main Article

Table 9

Inconclusive test results for human specimens with the US CDC real-time RT-PCR panel for detection of SARS-CoV-2*

Specimen ID Initial test, Ct
Retest, Ct
 Days after onset
N1 N2 N3 N1 N2 N3
Specimen from suspected cases
NP1
 Neg
 39.3
 Neg
Neg
 Neg
 36.9
 Unknown†
Serial follow-up specimens from laboratory-confirmed COVID-19 cases
NP1 36.1 39.8 Neg Neg Neg 37.5 15
NP2 39.6 39.6 Neg 36.9 38.5 Neg 16
NP3 41.6 Neg Neg 37.8 Neg 39.4 12
NP4 38.1 Neg Neg 37.0 38.5 Neg 14
NP5 Neg 37.8 36.1 Neg 38.3 36.8 16
NP6 Neg 40.1 Neg Neg 37.5 37.0 22
NP7 Neg 38.3 36.1 37.2 Neg Neg 13
NP8 38.3 Neg 36.0 36.7 38.1 Neg 22
NP9 Neg 39.9 Neg Neg 38.7 Neg 25
NP10 Neg Neg 36.4 Neg Neg 37.6 11
NP11 36.6 Neg 36.2 Neg 38.0 Neg 13
NP12 37.5 39.2 Neg Neg 38.9 36.0 15
NP13 36.9 Neg 39.5 Neg 36.9 37.0 13
NP14 36.4 40.2 Neg 36.2 Neg Neg 17
NP15 37.0 Neg Neg Neg 37.2 Neg 18
NP16 Neg 39.1 35.8 38.0 37.3 Neg 11
NP17 35.4 37.9 Neg Neg 38.7 36.9 16
NP18 Neg 37.8 36.1 36.7 37.2 Neg Unknown‡
NP19 35.5 39.0 Neg 39.8 Neg Neg Unknown‡
OP1 Neg 38.0 Neg 35.9 Neg Neg 18
OP2 36.3 38.2 Neg 38.4 Neg Neg 20
OP3 Neg 39.1 Neg Neg 38.4 37.8 11
OP4 Neg 38.2 37.3 35.8 Neg 36.0 10
OP5 Neg 37.2 36.7 37.7 Neg Neg 7
OP6 Neg Neg 36.5 38.1 Neg Neg 9
OP7 Neg Neg 38.4 Neg Neg 36.6 9
OP8 37.2 38.2 Neg 37.2 Neg 36.8 11
OP9 Neg 37.6 38.1 Neg Neg 39.3 15
OP10 36.6 38.0 Neg Neg 39.3 Neg 9
OP11 Neg Neg Neg Neg Neg Neg Unknown‡
OP12 Neg 37.8 Neg 37.6 Neg 37.6 Unknown‡
Sputum 1 Neg 42.9 37.3 Neg Neg 38.6 10
Sputum 2 38.0 Neg Neg 36.1 38.2 Neg 12
Serum 1 39.8 Neg Neg Neg 39.6 Neg 6
Serum 2 38.1 Neg Neg Neg Neg 35.8 16
Stool 1 Neg Neg 38.3 Neg 40.1 Neg 21
Stool 2 Neg 38.8 Neg 36.9 39.2 Neg 13
Stool 3 36.0 Neg Neg 37.3 Neg Neg 15
Stool 4 Neg 39.9 Neg 37.7 Neg 35.8 9

*CDC, Centers for Disease Control and Prevention; Ct, threshold cycle; COVID-19, coronavirus disease; N, nucleocapsid protein gene; neg, negative; NP, nasopharyngeal; OP, oropharyngeal; rRT-PCR, real-time reverse transcription PCR; SARS-CoV-2, severe acute respiratory coronavirus 2.
†Repatriated from Diamond Princess Cruise Ship, Japan.
‡Repatriated from Diamond Princess Cruise Ship and confirmed positive in Japan.

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Page created: May 14, 2020
Page updated: July 17, 2020
Page reviewed: July 17, 2020
The conclusions, findings, and opinions expressed by authors contributing to this journal do not necessarily reflect the official position of the U.S. Department of Health and Human Services, the Public Health Service, the Centers for Disease Control and Prevention, or the authors' affiliated institutions. Use of trade names is for identification only and does not imply endorsement by any of the groups named above.
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