US CDC Real-Time Reverse Transcription PCR Panel for Detection of Severe Acute Respiratory Syndrome Coronavirus 2
Xiaoyan Lu, Lijuan Wang, Senthilkumar K. Sakthivel, Brett Whitaker, Janna Murray, Shifaq Kamili, Brian Lynch, Lakshmi Malapati, Stephen A. Burke, Jennifer Harcourt, Azaibi Tamin, Natalie J. Thornburg, Julie M. Villanueva, and Stephen Lindstrom
Author affiliations: Centers for Disease Control and Prevention, Atlanta, Georgia, USA (X. Lu, B. Whitaker, S.A. Burke, J. Harcourt, A. Tamin, N.J. Thornburg, J.M. Villanueva, S. Lindstrom); Synergy America, Inc., Atlanta (L. Wang, S. Kamili); Eagle Contracting, Atlanta (S.K. Sakthivel, J. Murray, B. Lynch); Leidos, Atlanta (L. Malapati); Battelle, Atlanta (S.A. Burke)
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Table 2
Limits of detection of the US CDC rRT-PCR panel for detection of SARS-CoV-2 with RNA transcripts*
Copies/reaction |
No. positive tests/no. (%) reaction replicates
|
N1 |
N2 |
N3 |
20 |
24/24 (100) |
24/24 (100) |
24/24 (100) |
10 |
24/24 (100) |
24/24 (100) |
24/24 (100) |
5 |
24/24 (100) |
24/24 (100) |
24/24 (100) |
2.5 |
23/24 (95.8) |
16/24 (66.7) |
15/24 (62.5) |
1.25 |
15/24 (62.5) |
8/24 (33.3) |
3/24 (12.5) |
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Page created: May 14, 2020
Page updated: July 17, 2020
Page reviewed: July 17, 2020
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