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Volume 26, Number 8—August 2020

US CDC Real-Time Reverse Transcription PCR Panel for Detection of Severe Acute Respiratory Syndrome Coronavirus 2

Xiaoyan Lu, Lijuan Wang, Senthilkumar K. Sakthivel, Brett Whitaker, Janna Murray, Shifaq Kamili, Brian Lynch, Lakshmi Malapati, Stephen A. Burke, Jennifer Harcourt, Azaibi Tamin, Natalie J. Thornburg, Julie M. Villanueva, and Stephen LindstromComments to Author 
Author affiliations: Centers for Disease Control and Prevention, Atlanta, Georgia, USA (X. Lu, B. Whitaker, S.A. Burke, J. Harcourt, A. Tamin, N.J. Thornburg, J.M. Villanueva, S. Lindstrom); Synergy America, Inc., Atlanta (L. Wang, S. Kamili); Eagle Contracting, Atlanta (S.K. Sakthivel, J. Murray, B. Lynch); Leidos, Atlanta (L. Malapati); Battelle, Atlanta (S.A. Burke)

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Table 2

Limits of detection of the US CDC rRT-PCR panel for detection of SARS-CoV-2 with RNA transcripts*

Copies/reaction No. positive tests/no. (%) reaction replicates
N1 N2 N3
20 24/24 (100) 24/24 (100) 24/24 (100)
10 24/24 (100) 24/24 (100) 24/24 (100)
5 24/24 (100) 24/24 (100) 24/24 (100)
2.5 23/24 (95.8) 16/24 (66.7) 15/24 (62.5)
1.25 15/24 (62.5) 8/24 (33.3) 3/24 (12.5)

*CDC, Centers for Disease Control and Prevention; N, nucleocapsid protein gene; rRT-PCR, real-time reverse transcription PCR; SARS-CoV-2, severe acute respiratory coronavirus 2.

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Page created: May 14, 2020
Page updated: July 17, 2020
Page reviewed: July 17, 2020
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