Volume 26, Number 8—August 2020
Synopsis
US CDC Real-Time Reverse Transcription PCR Panel for Detection of Severe Acute Respiratory Syndrome Coronavirus 2
Xiaoyan Lu, Lijuan Wang, Senthilkumar K. Sakthivel, Brett Whitaker, Janna Murray, Shifaq Kamili, Brian Lynch, Lakshmi Malapati, Stephen A. Burke, Jennifer Harcourt, Azaibi Tamin, Natalie J. Thornburg, Julie M. Villanueva, and Stephen Lindstrom
Table 7
Cross-reactivity of the US CDC rRT-PCR panel for detection of SARS-CoV-2 against other respiratory pathogens*
| Pathogen (strain) | Source | Other respiratory pathogens, rRT-PCR (Ct) | SARS-CoV-2 rRT-PCR |
||
|---|---|---|---|---|---|
| N1 | N2 | N3 (Ct) | |||
| Adenovirus C1 (Ad.71) | Virus isolate | Pos (14.0) | Neg | Neg | Neg |
| Bocavirus | Clinical specimen | Pos (14.9) | Neg | Neg | Neg |
| Coronavirus 229E | Virus isolate | Pos (9.6) | Neg | Neg | Neg |
| Coronavirus OC43 | Virus isolate | Pos (12.9) | Neg | Neg | Neg |
| Coronavirus HKU1 | Clinical specimen | Pos (22.3) | Neg | Neg | Neg |
| Coronavirus MERS | Virus isolate | Pos (14.3) | Neg | Neg | Neg |
| Coronavirus NL63 | Clinical specimen | Pos (21.9) | Neg | Neg | Neg |
| Coronavirus SARS (Urbani) | Virus isolate | Pos (27.3) | Neg | Neg | Pos (26.3)† |
| Enterovirus D68 | Virus isolate | Pos (21.3) | Neg | Neg | Neg |
| Human metapneumovirus (CAN 99–81) | Virus isolate | Pos (13.8) | Neg | Neg | Neg |
| Influenza A H1N1 (A/India/2012) | Virus isolate | Pos (14.7) | Neg | Neg | Neg |
| Influenza A H3N1 (A/Texas/2012) | Virus isolate | Pos (10.7) | Neg | Neg | Neg |
| Influenza B (B/Massachusetts/1999) | Virus isolate | Pos (8.4) | Neg | Neg | Neg |
| Parainfluenza 1 (C35) | Virus isolate | Pos (17.2) | Neg | Neg | Neg |
| Parainfluenza 2 (Greer) | Virus isolate | Pos (17.1) | Neg | Neg | Neg |
| Parainfluenza 3 (C-43) | Virus isolate | Pos (20.4) | Neg | Neg | Neg |
| Parainfluenza 4a (M-25) | Virus isolate | Pos (16.7) | Neg | Neg | Neg |
| Parainfluenza 4b (CH 19503) | Virus isolate | Pos (18.2) | Neg | Neg | Neg |
| Respiratory syncytial virus (Long) | Virus isolate | Pos (15.1) | Neg | Neg | Neg |
| Rhinovirus 1A | Virus isolate | Pos (15.9) | Neg | Neg | Neg |
| Mycoplasma pneumoniae | Cultured bacteria | Pos (20.7) | Neg | Neg | Neg |
| Streptococcus pneumoniae | Cultured bacteria | Pos (21.1) | Neg | Neg | Neg |
*CDC, Centers for Disease Control and Prevention; Ct, cycle threshold; neg, negative;pos, positive; MERS, Middle East respiratory syndrome; N, nucleocapsid protein gene; rRT-PCR, real-time reverse transcription PCR; SARS-CoV-2, severe acute respiratory coronavirus 2.
†N3 assay designed for universal detection of clade 2 and 3 within Sarbecovirus subgenus including SARS-CoV-2, SARS-CoV, and bat- and civet-SARS–like coronaviruses.
Page created: May 14, 2020
Page updated: July 17, 2020
Page reviewed: July 17, 2020
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