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Volume 26, Number 8—August 2020

Synopsis

US CDC Real-Time Reverse Transcription PCR Panel for Detection of Severe Acute Respiratory Syndrome Coronavirus 2

Xiaoyan Lu, Lijuan Wang, Senthilkumar K. Sakthivel, Brett Whitaker, Janna Murray, Shifaq Kamili, Brian Lynch, Lakshmi Malapati, Stephen A. Burke, Jennifer Harcourt, Azaibi Tamin, Natalie J. Thornburg, Julie M. Villanueva, and Stephen LindstromComments to Author 
Author affiliations: Centers for Disease Control and Prevention, Atlanta, Georgia, USA (X. Lu, B. Whitaker, S.A. Burke, J. Harcourt, A. Tamin, N.J. Thornburg, J.M. Villanueva, S. Lindstrom); Synergy America, Inc., Atlanta (L. Wang, S. Kamili); Eagle Contracting, Atlanta (S.K. Sakthivel, J. Murray, B. Lynch); Leidos, Atlanta (L. Malapati); Battelle, Atlanta (S.A. Burke)

Main Article

Table 3

Limits of detection of the US CDC rRT-PCR panel for detection of SARS-CoV-2 with extracted SARS-CoV-2 virus RNA*

Virus concentration, TCID50 N1 Ct
 N2 Ct
 N3 Ct
Test 1 Test 2 Test 3 Call rate Test 1 Test 2 Test 3 Call rate Test 1 Test 2 Test 3 Call rate
1 × 100.5 27.5 27.1 27.4 3/3 29.2 28.9 28.7 3/3 28.3 28.3 28.2 3/3
1 × 100 30.9 29.4 29.9 3/3 31.2 31.1 31.1 3/3 30.0 30.0 30.0 3/3
1 × 10−0.5 30.7 30.9 31.1 3/3 33.0 32.7 32.3 3/3 31.4 31.4 32.5 3/3
1 × 10−1 33.0 32.4 32.9 3/3 34.4 34.3 34.7 3/3 34.6 32.3 33.3 3/3
1 × 10−1.5 34.4 33.6 35.6 3/3 36.3 36.1 37.6 3/3 35.4 35.8 35.6 3/3
1 × 10−2 37.2 36.1 Neg 2/3 39.0 Neg Neg 1/3 36.1 Neg Neg 1/3
1 × 10-2.5 36.2 36.3 Neg 2/3 38.8 37.6 Neg 2/3 37.1 Neg Neg 1/3
1 × 10−3 Neg Neg Neg 0/3 Neg Neg Neg 0/3 Neg Neg Neg 0/3

*Call rate indicates each assay performed in triplicate. CDC, Centers for Disease Control and Prevention; Ct, cycle threshold; N, nucleocapsid protein gene; neg, negative; rRT-PCR, real-time reverse transcription PCR; SARS-CoV-2, severe acute respiratory coronavirus 2; TCID50, 50% tissue culture infectious dose.

Main Article

Page created: May 14, 2020
Page updated: July 17, 2020
Page reviewed: July 17, 2020
The conclusions, findings, and opinions expressed by authors contributing to this journal do not necessarily reflect the official position of the U.S. Department of Health and Human Services, the Public Health Service, the Centers for Disease Control and Prevention, or the authors' affiliated institutions. Use of trade names is for identification only and does not imply endorsement by any of the groups named above.
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