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Volume 27, Number 5—May 2021
Research

Engineered NS1 for Sensitive, Specific Zika Virus Diagnosis from Patient Serology

Thai Leong Yap1, Shin Yee Hong1, Jun Hui Soh, Lekha Ravichandraprabhu, Vanessa W.X. Lim, Hsi-Min Chan, Tommy Z.X. Ong, Ying Ping Chua, Shi En Koh, Huajing Wang, Yee Sin Leo, Jackie Y. Ying, and William SunComments to Author 
Author affiliations: Experimental Drug Development Centre, Singapore (T.L. Yap, S.Y. Hong); Institute of Bioengineering and Nanotechnology, Singapore (T.L. Yap, S.Y. Hong, L. Ravichandraprabhu, T.Z.X. Ong, Y.P. Chua, S.E. Koh, H. Wang, W. Sun); NanoBio Lab, Singapore (J.H. Soh, H.-M. Chan, J.Y. Ying); National Centre for Infectious Diseases, Singapore (V.W.X. Lim, Y.S. Leo); Tan Tock Seng Hospital, Singapore (Y.S. Leo); Yong Loo Lin School of Medicine, National University of Singapore (Y.S. Leo, W. Sun).

Main Article

Figure 3

H-zMut2 ELISA for validation set in study of Zika diagnosis, Singapore. A, D) H-zMut2 reactivity to IgM (A) and IgG (D) present in plasma collected during acute and recent convalescent phases (ZIKV-A, n = 70 [1–6 dpo]; ZIKV-C, n = 48 [7–14 dpo]; DENV-A, n = 81 [1–6 dpo]; DENV-B, n = 70 [7–21 dpo]). Plasma samples were blinded and tested with H-zMut2 as the capture antigen. Normalized OD >1.5 for plasma sample was determined as positive for ZIKV infection. Results are representative of 2 replicates for each plasma sample. B, C, E, F) Patient samples for ZIKV (B, E) and DENV (C, F). The plots show distribution of number of plasma cases (x-axis) over number of days post infection (y-axis, dpo) for H-zMut2 ELISA tested with validation set; the number of positive plasma samples (black bar) was shown against the total (gray bar) for each dpo. DENV, dengue virus; dpo, days postonset of symptoms; OD, optical density; ZIKA, Zika virus.

Figure 3. H-zMut2 ELISA for validation set in study of Zika diagnosis, Singapore. A, D) H-zMut2 reactivity to IgM (A) and IgG (D) present in plasma collected during acute and recent convalescent phases (ZIKV-A, n = 70 [1–6 dpo]; ZIKV-C, n = 48 [7–14 dpo]; DENV-A, n = 81 [1–6 dpo]; DENV-B, n = 70 [7–21 dpo]). Plasma samples were blinded and tested with H-zMut2 as the capture antigen. Normalized OD >1.5 for plasma sample was determined as positive for ZIKV infection. Results are representative of 2 replicates for each plasma sample. B, C, E, F) Patient samples for ZIKV (B, E) and DENV (C, F). The plots show distribution of number of plasma cases (x-axis) over number of days post infection (y-axis, dpo) for H-zMut2 ELISA tested with validation set; the number of positive plasma samples (black bar) was shown against the total (gray bar) for each dpo. DENV, dengue virus; dpo, days postonset of symptoms; OD, optical density; ZIKA, Zika virus.

Main Article

1These authors contributed equally to this article.

Page created: April 05, 2021
Page updated: April 20, 2021
Page reviewed: April 20, 2021
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