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Volume 27, Number 5—May 2021
Research

Engineered NS1 for Sensitive, Specific Zika Virus Diagnosis from Patient Serology

Thai Leong Yap1, Shin Yee Hong1, Jun Hui Soh, Lekha Ravichandraprabhu, Vanessa W.X. Lim, Hsi-Min Chan, Tommy Z.X. Ong, Ying Ping Chua, Shi En Koh, Huajing Wang, Yee Sin Leo, Jackie Y. Ying, and William SunComments to Author 
Author affiliations: Experimental Drug Development Centre, Singapore (T.L. Yap, S.Y. Hong); Institute of Bioengineering and Nanotechnology, Singapore (T.L. Yap, S.Y. Hong, L. Ravichandraprabhu, T.Z.X. Ong, Y.P. Chua, S.E. Koh, H. Wang, W. Sun); NanoBio Lab, Singapore (J.H. Soh, H.-M. Chan, J.Y. Ying); National Centre for Infectious Diseases, Singapore (V.W.X. Lim, Y.S. Leo); Tan Tock Seng Hospital, Singapore (Y.S. Leo); Yong Loo Lin School of Medicine, National University of Singapore (Y.S. Leo, W. Sun).

Main Article

Figure 7

ELISA for ZIKV NS1 detection in study of Zika diagnosis, Singapore. A) Receiver operating characteristics curve analysis showing the performance of C12-C11 sandwich ELISA when tested against ZIKV-infected or non–ZIKV-infected samples. B) ZIKV NS1 quantification in patient samples using in-house antibody pairs. Each dot represents an individual patient sample. C) Distribution of number of plasma cases (x-axis) over dpo (y-axis) for ZIKV NS1 ELISA tested with the validation set; positive plasma (black) and the total plasma cases (gray) at each dpo are also shown. DENV, dengue virus; dpo, days postonset of symptoms; NS1, nonstructural protein 1; ZIKV, Zika virus.

Figure 7. ELISA for ZIKV NS1 detection in study of Zika diagnosis, Singapore. A) Receiver operating characteristics curve analysis showing the performance of C12-C11 sandwich ELISA when tested against ZIKV-infected or non–ZIKV-infected samples. B) ZIKV NS1 quantification in patient samples using in-house antibody pairs. Each dot represents an individual patient sample. C) Distribution of number of plasma cases (x-axis) over dpo (y-axis) for ZIKV NS1 ELISA tested with the validation set; positive plasma (black) and the total plasma cases (gray) at each dpo are also shown. DENV, dengue virus; dpo, days postonset of symptoms; NS1, nonstructural protein 1; ZIKV, Zika virus.

Main Article

1These authors contributed equally to this article.

Page created: April 05, 2021
Page updated: April 20, 2021
Page reviewed: April 20, 2021
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