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Volume 27, Number 5—May 2021
Research

Engineered NS1 for Sensitive, Specific Zika Virus Diagnosis from Patient Serology

Thai Leong Yap1, Shin Yee Hong1, Jun Hui Soh, Lekha Ravichandraprabhu, Vanessa W.X. Lim, Hsi-Min Chan, Tommy Z.X. Ong, Ying Ping Chua, Shi En Koh, Huajing Wang, Yee Sin Leo, Jackie Y. Ying, and William SunComments to Author 
Author affiliations: Experimental Drug Development Centre, Singapore (T.L. Yap, S.Y. Hong); Institute of Bioengineering and Nanotechnology, Singapore (T.L. Yap, S.Y. Hong, L. Ravichandraprabhu, T.Z.X. Ong, Y.P. Chua, S.E. Koh, H. Wang, W. Sun); NanoBio Lab, Singapore (J.H. Soh, H.-M. Chan, J.Y. Ying); National Centre for Infectious Diseases, Singapore (V.W.X. Lim, Y.S. Leo); Tan Tock Seng Hospital, Singapore (Y.S. Leo); Yong Loo Lin School of Medicine, National University of Singapore (Y.S. Leo, W. Sun).

Main Article

Figure 2

Reactivity of nonstructural protein 1 antigens to ZIKV and DENV plasma in study of Zika diagnosis, Singapore. H-zMut2 ELISA was tested with a training set for binding to IgM (A) and IgG (B). Results are representative of replicates for each sample. Normalized OD >1.5 for plasma or serum sample was determined as positive for ZIKV infection. DENV, dengue virus; OD, optical density; ZIKA, Zika virus.

Figure 2. Reactivity of nonstructural protein 1 antigens to ZIKV and DENV plasma in study of Zika diagnosis, Singapore. H-zMut2 ELISA was tested with a training set for binding to IgM (A) and IgG (B). Results are representative of replicates for each sample. Normalized OD >1.5 for plasma or serum sample was determined as positive for ZIKV infection. DENV, dengue virus; OD, optical density; ZIKA, Zika virus.

Main Article

1These authors contributed equally to this article.

Page created: April 05, 2021
Page updated: April 20, 2021
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